摘要
目的分析肾结核与肾肿瘤患者血清以及尿液中甲壳质酶蛋白-40(YKL-40)水平和差异。方法选择2016年5月—2019年5月河北北方学院附属第一医院(以下简称“我院”)收治的100例肾结核患者作为肾结核组,50例肾肿瘤患者作为肾肿瘤组。另选择同期来我院进行健康体检的100名体检者作为参照组,利用酶联免疫吸附测定法分别检测三组血清以及尿液中YKL-40水平,同时采集肾结核以及肾肿瘤患者病理组织和病变周围,将其制成石蜡标本,采取免疫组化分析法观察病变组织中YKL-40水平。回顾性分析全部研究样本临床资料,比较肾结核以及肾肿瘤患者与正常体检者尿液、血清中YKL-40水平差异;观察肾结核以及肾肿瘤患者病变组织与周围正常组织中YKL-40表达情况。结果参照组血清以及尿液标本中YKL-40水平低于肾结核组、肾肿瘤组,差异有统计学意义(P<0.05)。肾结核组与肾肿瘤组血清以及尿液中YKL-40水平比较,差异无统计学意义(P>0.05)。肾结核以及肾肿瘤组病变组织中YKL-40水平明显高于周围正常组织,差异有统计学意义(P<0.05)。结论肾结核以及肾肿瘤患者尿液、血清中YKL-40水平有明显升高,可将其作为疾病初步诊断依据,但为提升诊断准确性,还需与其他实验室诊断标准相结合。
Objective To analysis the levels and differences of chitinase protein-40(YKL-40)in serum and urine of patients with renal tuberculosis and renal tumors.Methods From May 2016 to May 2019,100 patients with renal tuberculosis admitted to the First Affiliated Hospital of Hebei North University(hereinafter referred to as“our hospital”)were selected as renal tuberculosis group and 50 patients with renal tumor as renal tumor group.Meanwhile,100 examinees who came to our hospital for physical examination at the same time were selected as the reference group,and YKL-40 levels in serum and urine of three groups of patients were detected by enzyme-linked immunosorbent assay.At the same time,the normal tissues around the lesion were collected and made into paraffin samples.The expression of YKL-40 in the lesion was observed by immunohistochemistry.The clinical data of all study samples were retrospectively analyzed.Compared the difference of YKL-40 levels in urine and serum between patients with renal tuberculosis and renal tumor and normal persons,and observed the expression of YKL-40 in pathological tissues and surrounding normal tissues of patients with renal tuberculosis and renal tumor.Results The level of YKL-40 in serum and urine samples of reference group was lower than that of renal tuberculosis group and renal tumor group(P<0.05).There was no significant difference in serum and urine YKL-40 levels between renal tuberculosis group and renal tumor group(P>0.05).The level of YKL-40 in pathological tissues of patients with renal tuberculosis and renal tumor was significantly higher than that in surrounding normal tissues(P<0.05).Conclusion The level of YKL-40 in urine and serum of patients with renal tuberculosis and renal tumor is significantly increased,which can be used as the basis for the preliminary diagnosis of the disease.However,in order to improve the diagnostic accuracy,it should be combined with other laboratory diagnostic criteria.
作者
李秀君
安海霞
杨家鑫
陈怀安
LI Xiujun;AN Haixia;YANG Jiaxin;CHEN Huai’an(Department of Urology,the First Affiliated Hospital of Hebei North University,Hebei Province,Zhangjiakou 075000,China)
出处
《中国医药导报》
CAS
2021年第6期95-98,共4页
China Medical Herald
基金
河北省医学科学研究重点课题计划项目(20200506)
河北省张家口科技攻关项目(1921049D)。
关键词
肾结核
肾肿瘤
血尿
贫血
甲壳质酶蛋白-40
Renal tuberculosis
Renal tumor
Hematuria
Anemia
Chitinase protein-40
作者简介
通讯作者:陈怀安(1979.1-),男,硕士,副主任医师,副教授,研究方向:泌尿系肿瘤的诊治。
