摘要
为了探明茶树菇有性生殖内在分子机制,包括交配极性转换产生的同核体出菇问题。以茶树菇YSG同核体子代为实验菌株,依据基因组注释信息,利用嵌套引物验证B交配位点,设计引物扩增同核体群体,获得的扩增谱与菌株交配型进行比对。结果表明:(1)同核体培养后出现3种类型,菌丝型、无盖型和菌柄型,后两种为畸形子实体,不进行有性生殖;(2)依据交配位点B设计的引物扩增谱显示,B交配位点完整遗传到子代菌株中,没有观察到重组事件发生;(3)交配型分析显示,该B交配位点决定菌株的交配型,没有新的交配极性出现。本研究推断供试菌株茶树菇YSG同核体只包含一个B交配位点,结果与已报道的3个亚位点不同。这些结果差异是否由于类群差异造成,还需要更多的出菇实验及交配位点鉴定予以确认。
The aim is to investigate the molecular mechanism of sexual reproduction including mating type switching in Agrocybe aegerita.Homokaryon offspring isolated from Agrocybe aegerita YSG strain was employed in this study.Based on the genome annotation,B mating locus was identified using overlapping primers.Primer pairs for B mating genes were used for amplifying the monokaryotic population,and the amplification spectrum was compared with mating type.The results showed:(1)three types including mycelia,capless and stripe-like fruiting bodies occurred after the cultivation of homokaryon,and the latter two were the abnormal fruiting bodies without going through the sexual reproduction;(2)the amplified spectrum using the primer pairs based on the mating gene sequences showed that the complete B mating locus was inherited into the offspring strains without recombination event occurrence;(3)mating type analysis showed that B mating locus governed the mating behavior,and new mating type was not found.It is inferred that Agrocybe aegerita YSG only contained one B mating locus,which is also different from the three reported sublocus.More fruiting tests and mating loci identification are needed for clarifying whether the differences are caused by the group varieties.
作者
钱蓉
陈卫民
柴红梅
陶南
赵永昌
陈玉惠
Qian Rong;Chen Weimin;Chai Hongmei;Tao Nan;Zhao Yongchang;Chen Yuhui(College of Life Science Southwest Forestry University,Kunming 650224;Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650221;Yunnan Provincial Key Lab of Agricultural Biotechnology,Kunming 650223;Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation,Ministry of Agriculture,Kunming 650223)
出处
《中国农学通报》
2021年第2期14-19,共6页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金“信息素受体多等位基因在田头菇属真菌生物种识别中的作用研究”(31360024)
现代农业(食用菌)产业技术体系(CARS20)。
关键词
茶树菇
同核体出菇
B交配基因
交配型转换
有性生殖
Agrocybe aegerita
homokaryon fruiting
B mating gene
mating type switching
sexual reproduction
作者简介
第一作者:钱蓉,女,1993年出生,云南曲靖人,硕士研究生,主要从事大型食用真菌遗传学方面的研究。通信地址:650205昆明市盘龙区北京路延长线2238号云南省农业科学院生物技术与种质资源研究所,Tel:0871-65174681,E-mail:1433549562@qq.com;通讯作者:陈玉惠,女,1961年出生,云南保山人,教授,博士,主要从事真菌资源学方面的研究。通信地址:650224昆明市盘龙区白龙寺300号西南林业大学,E-mail:cyh196107@126.com。
