摘要
为获得高性能的金龟子绿僵菌菌株,本研究采用常压室温等离子体(Atmospheric and room temperature plasma,ARTP)和紫外线复合诱变处理金龟子绿僵菌,通过比较产孢快慢和生长直径大小进行初筛,用抗紫外能力和毒力作为复筛指标选育菌株,并对育种获得的突变株进行耐热性和遗传稳定性试验,最终在ARTP 40 s和紫外120 s时筛选出一株产孢量高、耐紫外线、毒力强、遗传稳定的优良菌株AU34。该菌株产孢时间比原始菌株快,产孢量为1.63±0.22(108 cell/cm^2),较原始菌株提高了63.81%。经紫外照射5 min后,AU34存活率为3.18%,比原始菌株更耐紫外照射。并且AU34对小菜蛾的毒力强,校正死亡率为85.71%,半数致死时间(Half-lethal time,LT 50)为6.12 d。与原始菌株相比,AU34的耐热性也有较大提高。传代培养6代后,菌株AU34的产孢量无明显变化,具有良好的遗传稳定性。
After atmospheric and room temperature plasma(ARTP)and ultraviolet(UV)compound mutation,a high-performance strains of Metarhizium Anisopliae was selected.By comparing the speed of sporulation and the size of growth diameter,selected with the ability of anti-ultraviolet and virulence as the re-screening index,and tested for heat resistance and genetic stability,a good strain AU34 with high sporulation,UV tolerance,strong virulence and stable genetic was selected at the time of ARTP 40 s and UV 120 s.The sporulation time of the strain was faster than the original strain,and the sporulation amount was 1.63±0.22(108 cell/cm^2),which was 63.81%higher than the original strain.After 5 min of UV irradiation,the survival rate of AU34 was 3.18%,which was more resistant to UV irradiation than the original strain.The toxicity of AU34 to Plutella xylostella L.was high.The adjusted mortality rate was 85.71%and LT 50 was 6.12 days.Compared with the original strain,the heat resistance of AU34 was also improved.After subculture for 6 generations,the sporulation of strain AU34 had no obvious change and had good genetic stability.
作者
黄玉
尼玛扎西
薛正莲
张国强
HUANG Yu;Nimazhaxi;XUE Zhenglian;ZHANG Guoqiang(College of Biological and Chemical Engineering,Anhui Polytechnic University,Wuhu 241000,China;Institute of Resources and Environment,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa 850000,China)
出处
《食品工业科技》
CAS
北大核心
2021年第4期60-64,70,共6页
Science and Technology of Food Industry
基金
中央引导地方资金(Y20X20195400004790)
平台引进高层次人才资助项目(2018PTJB03)
安徽省高校学科拔尖人才项目(jxbjZD22)。
关键词
金龟子绿僵菌
常压室温等离子体(ARTP)
紫外诱变
复合诱变
筛选
遗传稳定性
Metarhizium anisopliae
atmospheric and room temperature plasma(ARTP)
ultraviolet mutagenesis
compound mutation
screening
genetic stability
作者简介
黄玉(1995-),女,硕士研究生,研究方向:微生物发酵工程,E-mail:1553067871@qq.com;并列第一作者:尼玛扎西(1973-),男,本科,副研究员,研究方向:资源与环境生物技术,E-mail:nyima313@163.com;通信作者:张国强(1982-),男,博士,教授,研究方向:农业与食品生物技术,E-mail:guoqiang2008@163.com。
