摘要
【目的】筛选能降解难溶性磷的PGPR菌株。【方法】采用解磷圈法从15株PGPR中筛选透明圈直径最大的菌株,并将解磷菌加入到番茄育苗基质和灌根方式测定了解磷菌对幼苗期和成株期番茄促生作用及根围土壤养分的影响。【结果】经过16srRNA序列比对,2株解磷菌LZT-5和FQG-5分别为不动杆菌(Acinetobacter)和嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia),其解磷透明圈直径分别为2.33和2.27 cm。用10%FQG-5和LZT-5育苗后番茄幼苗株高、茎粗和叶面积最高,分别为13.66 cm、0.35 cm、28.95 cm2和13.70 cm、0.36 cm、29.75 cm2,显著高于其他处理。用FQG-5灌根后露地番茄根围的速效磷、速效钾、硝态氮、有机质、全氮、全磷、全钾含量分别为40.05 mg·kg^-1、193.00 mg·kg^-1、78.30 mg·kg^-1、50.53 mg·kg^-1、37.79 g·kg^-1、0.80 g·kg^-1、0.79 g·kg^-1和2.03 g·kg^-1,均显著高于其他处理。【结论】解磷菌FQG-5可以促进番茄根围土壤养分转化成速效性养分,从而促进番茄植株生长,提高产量。
【Objective】This paper aimed to find out the strains that could degrade insoluble phosphorus in the soil.【Method】The phosphate solubilizing method was used to select the strains in 15 PGPR strains, and the effect of phosphorus bacteria on the growth promotion of seedlings and adult tomatoes and on the soil nutrients in the rhizosphere by adding the phosphate bacteria to the tomato seedling substrate and root irrigation was determined.【Result】The diameters of the transparent circle of LZT-5 and FQG-5 which were identified as Acinetobacter and Stenotrophomonas maltophilia with 16 sRNA sequence blasting were 2.33 and 2.27 cm, respectively. The plant height, stem diameter and leaf area of tomato seedlings were the highest, which were 13.66 cm, 0.35 cm, 28.95 cm2 and 13.70 cm, 0.36 cm and 29.75 cm2, respectively, dramatically raised with 10 % FQG-5 and LZT-5, being significantly higher than that with other treatments. The available P, K, NO3-N, organic matter, total N, total P and total K in the tomato rhizosphere were 40.05 mg·kg^-1, 193.00 mg·kg^-1, 78.30 mg·kg^-1, 50.53 mg·kg^-1, 37.79 g·kg^-1, 0.80 g·kg^-1, 0.79 g·kg^-1 and 2.03 g·kg^-1, respectively, being significantly higher than that in other treatments. 【Conclusion】Therefore, it could be concluded that FQG-5 in the tomato rhizosphere could enhance the soil nutrients transformation into available nutrients and promote tomato growth.
作者
贺字典
高玉峰
王燕
李翠霞
高歆瑶
张志浩
HE Zi-dian;GAO Yu-feng;WANG Yan;LI Cui-xia;GAO Xin-yao;ZHANG Zhi-hao(Hebei Normal University of Science&Technology,Hebei Qinhuangdao 066004,China;Agricultural and Rural Bureau of Lulong County,Hebei Lulong 066400,China;Cangzhou Development Zone Central School,Hebei Cangzhou 061000,China)
出处
《西南农业学报》
CSCD
北大核心
2020年第12期2891-2896,共6页
Southwest China Journal of Agricultural Sciences
基金
河北省重点研发计划项目农业关键共性技术攻关专项(18226507D)
河北省自然基金(C2019407103)
河北省高等学校科学技术研究重点项目(ZD2019018)
河北省蔬菜产业体系病虫害绿色防控岗位项目(HBCT2018030207)。
关键词
植物根际促生菌
促生作用
解磷效果
生长性状
Plant growth promoting rhizobacteria(PGPR)
Plant growth promoting effect
Phosphate-solubilizing effect
Growth traits
作者简介
贺字典(1972-),女,河北任丘人,教授,博士,硕士生导师,主要从事蔬菜和中草药病害生物防治研究;通讯作者:高玉峰(1972-),男,河北抚宁人,研究员,硕士,硕士生导师,主要从事病害生物防治研究。
