摘要
目的观察3种丹参注射剂对大鼠急性心肌缺血的改善作用。方法将90只SD大鼠按照随机数字表法分为6组,正常组、模型组、注射用丹参(冻干)组、丹参注射液、注射用丹参多酚酸盐组、硝酸甘油注射液组,每组15只。注射用丹参(冻干)组大鼠予注射用丹参(冻干) 4 mg/m L腹腔注射;丹参注射液组大鼠予丹参注射液4 mg/m L腹腔注射;注射用丹参多酚酸盐组大鼠予注射用丹参多酚酸盐1.6 mg/m L腹腔注射;硝酸甘油注射液组大鼠予硝酸甘油注射液0.5 mg/m L腹腔注射。实验药物剂量为成人常用剂量按照人鼠体表面积换算公式换算成等效剂量,各药物临用前用0.9%氯化钠注射液配制成所需浓度,给药容积为10 m L/kg。正常组、模型组大鼠予等容积0.9%氯化钠注射液腹腔注射。均每日1次,连续给药3 d。第3 d给药后1 h,各组大鼠均麻醉后测标准肢体Ⅱ导联心电图作为急性心肌缺血前值。然后注射用丹参(冻干)组、丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠制备急性心肌缺血模型,监测标准肢体Ⅱ导联心电图15 min,采集急性心肌缺血后1、5、10、15 min的心电图为急性心肌缺血后值。观察各组大鼠急性心肌缺血后1、5、10、15 min心电图J点位移变化、T波高度变化;比较各组大鼠血清肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)变化。结果模型组大鼠急性心肌缺血后1、5、10、15 min心电图J点位移变化、T波高度变化均高于正常组(P<0.05),说明急性心肌缺血模型成功;注射用丹参(冻干)组、丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠急性心肌缺血后5、10、15 min心电图J点位移变化、T波高度变化均低于模型组(P<0.05);注射用丹参(冻干)组大鼠急性心肌缺血后10、15 min心电图J点位移变化、T波高度变化均低于丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组(P<0.05);丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠急性心肌缺血后10、15 min心电图J点位移变化、T波高度变化两两比较差异均无统计学意义(P>0.05);注射用丹参(冻干)组、丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠急性心肌缺血后1、5 min心电图J点位移变化、T波高度变化两两比较差异均无统计学意义(P>0.05)。模型组大鼠血清CK、CK-MB、LDH均高于正常组(P<0.05),说明急性心肌缺血模型成功;注射用丹参(冻干)组大鼠血清CK、CK-MB、LDH均低于模型组(P<0.05);丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠血清CK-MB、LDH均低于模型组(P<0.05);注射用丹参(冻干)组大鼠血清CK、CK-MB均低于丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组(P<0.05);丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠血清CK、CK-MB两两比较差异均无统计学意义(P>0.05);注射用丹参(冻干)组、丹参注射液组、注射用丹参多酚酸盐组、硝酸甘油注射液组大鼠LDH两两比较差异均无统计学意义(P>0.05)。结论 3种丹参注射剂对大鼠急性心肌缺血均有一定的保护作用,注射用丹参(冻干)的改善作用略优于丹参注射液、注射用丹参多酚酸盐,其作用差异可能主要与丹参素、丹酚酸A及丹酚酸B含量不同有关。
Objective To observe the improvement of Salvia miltiorrhiza injection on acute myocardial ischemia in rats. Methods 90 SD rats were randomly divided into 6 groups: normal group, model group, Salvia miltiorrhiza lyophilized powder group, Salvia miltiorrhiza parenteral solution group,Salvianolate injection group and nitroglycerin injection group,with 15 rats in each group.Rats in Salvia miltiorrhiza lyophilized powder group were intraperitoneally injected with 4 mg/m L Salvia miltiorrhiza lyophilized powder injection;rats in Salvia miltiorrhiza parenteral solution group were intraperitoneally injected with 4 mg/m L Salvia miltiorrhiza injection;rats in Salvianolate injection group were intraperitoneally injected with 1. 6 mg/m L salvianolate injection;rats in nitroglycerin injection group were intraperitoneally injected with 0. 5 mg/m L nitroglycerin injection. The dosage of the experimental drug was converted into equivalent dose according to dosage commonly used in adults on the basis of the conversion formula of human and mouse body surface area. Before clinical use,the required concentration of each drug was prepared with 0. 9% sodium chloride injection,and the administration volume was 10 m L/kg. The rats in normal group and model group were injected intraperitoneally with equal volume of 0. 9% sodium chloride injection. They were given injection once a day for 3 days. One hour after administration on the third day,the standard II lead ECG was measured as the pre-value of acute myocardial ischemia. Then the acute myocardial ischemia model was established in the four drug administration groups. The standard II lead ECG was monitored for 15 min,and the ECG at 1,5,10 and 15 min after acute myocardial ischemia was collected as the value after acute myocardial is chemia. The changes of J-point displacement and T-wave height of ECG at 1,5,10 and 15 min after acute myocardial ischemia were observed,and the changes of serum creatine kinase( CK),creatine kinase is oenzyme( CK-MB) and lactate dehydrogenase( LDH) were compared. Results The changes of J point displacement and T wave height at 1,5,10,15 min after acute myocardial ischemia in the model group were higher than those in the normal group( P < 0. 05),indicating that the acute myocardial ischemia model was successful. The changes of ECG J-point displacement and T-wave height at 5,10 and 15 min after acute myocardial ischemia in the Danshen injection( lyophilized) group,Danshen injection group,salvianolate injection group and nitroglycerin injection group were lower than those in the model group( P < 0. 05). The changes of ECG J point displacement and T wave height at 10 and 15 min after acute myocardial ischemia in the Danshen injection( lyophilized)group were lower than those in the Danshen injection group,the salvianolate injection group and the nitroglycerin injection group( P < 0. 05). There was no statistically significant difference in the J-point displacement and T-wave height of ECG at 10 and 15 min after acute myocardial ischemia in rats of Danshen injection group,salvianolate injection group and nitroglycerin injection group( P > 0. 05). There was no significant difference in the J-point displace ment and T-wave height of ECG at 1 and 5 min after acute myocardial ischemia in rats of Danshen injection( lyophilized) group,Danshen injection group,salvianolate injection group and nitroglycerin injection group( P > 0. 05).The serum CK,CK-MB and LDH in the model group were higher than those in the normal group( P < 0. 05),indicating that the acute myocardial ischemia model was successful. The serum CK,CK-MB and LDH levels in the Danshen injection( lyophilized) group were lower than those in the model group( P < 0. 05). The serum CK-MB and LDH in the Danshen injection group,salvianolate injection group and nitroglycerin injection group were lower than those in the model group( P < 0. 05). The serum CK and CK-MB of rats in Danshen injection( freeze-dried)group were lower than those in Danshen injection group,salvianolate injection group and nitroglycerin injection group( P < 0. 05). There was no significant difference in serum CK and CK-MB between Danshen injection group,salvianolate injection group and nitroglycerin injection group( P > 0. 05). There was no significant difference in LDH between Danshen injection( lyophilized) group,Danshen injection group,salvianolate injection group and nitroglycerin injection group( P > 0. 05). Conclusion Three kinds of Salvia miltiorrhiza injection have certain protective effect on acute myocardial ischemia in rats. Salvia miltiorrhiza lyophilized powder has a slightly better improvement than Salvia miltiorrhiza parenteral solution and Salvianolate injection. The difference of its effect may be mainly related to the different contents of Danshensu,salvianolic acid A and salvianolic acid B.
作者
张海啸
于长安
史载祥
杜金行
ZHANG Haixiao;YU Chang'an;SHI Zaixiang(Department of TCM,Chuiyangliu Hospital Affiliated to Tsinghua University,Beijing 100022)
出处
《河北中医》
2020年第11期1685-1689,1699,共6页
Hebei Journal of Traditional Chinese Medicine
关键词
心肌缺血
丹参
注射剂
动物实验
Myocardial ischemia
Salvia miltiorrhiza
Injections
Animal experiments
作者简介
张海啸(1972-),女,副主任医师,博士。从事中西医结合心血管疾病的基础与临床研究;通讯作者:史载祥。
