摘要
Malvidin-3-galactoside(Mv-3-gal)is the major anthocyanin monomer in blueberry anthocyanins.The compound is well-characterized by its anti-oncogenesis function in multiple organs including liver.In the current study,the mechanism driving the anti-hepatocellular carcinoma(HCC)function of Mv-3-gal was further explored by focusing on apoptosis and metastases pathways.HCC cell line Huh-7 was firstly administrated with Mv-3-gal of different concentrations and the effect of Mv-3-gal on cell proliferation,apoptosis,colony formation ability,metastasis potential as well as the activity of Akt/PTEN and MAPK pathways was assessed.The results showed that Mv-3-gal inhibited the cell proliferation and colony formation ability,induced cell cycle arrest and apoptosis in a dose-dependent manner.Regarding the metastasis potential,Mv-3-gal suppressed the migration and invasion potential of Huh-7 cells by regulating MMPs expression.Taken together,the findings highlighted the anti-HCC potent of Mv-3-gal,which was associated with the inhibition of Akt/PTEN,MAPK and MMP pathways.
基金
The work is thanks to supported by the national natural science foundation of China(31671863)
the national key research and development plan(2016YFD0400200)
innovative talent support program for institution of higher learning of Liaoning province(LR2017038)
young and middle-aged technological innovation talent support for program of Shenyang science and technology bureau(RC170247)
thanks to the technically supported by the General Hospital of the Shenyang Military Region of the Chinese People’s Liberation Army.
作者简介
Corresponding authors at:Bin Li,College of Food Science,Shenyang Agricultural University,Shenyang,Liaoning,G110866,PR China.E-mail addresses:libinsyau@163.com;Corresponding authors at:Yuehua WangCollege of Food Science,Shenyang Agricultural University,Shenyang,Liaoning,G110866,PR China.E-mail addresses:wangyuehua20122132@163.com。
