摘要
为了解越冬红嘴鸥及其栖息环境中部分动物弓形虫流行情况,采用间接血凝实验(IHA)检测红嘴鸥血清386份,并采用巢式PCR扩增弓形虫B1基因,包括检测红嘴鸥血样403份、抚仙湖、滇池等红嘴鸥栖息地环境中褐家鼠128只和散养土鸡34只。结果,弓形虫抗体阳性率为11.40%(44/386),弓形虫B1基因阳性检出率为9.03%(51/565),其中红嘴鸥为3.23%(13/403),褐家鼠为17.19%(22/128),散养土鸡为47.06%(16/34)。结果表明,红嘴鸥栖息环境中褐家鼠和散养土鸡存在弓形虫感染情况。该调查初步获悉了来滇越冬红嘴鸥及栖息地动物弓形虫流行情况,对弓形虫病防控具有公共卫生意义,为迁徙候鸟弓形虫病溯源、预防、诊疗提供了参考。
We studied the prevalence of Toxoplasma gondii in wintering black-headed gulls and some animals in their habitats.We analyzed 386 samples of black-headed gull serum by use of indirect haemagglutination(IHA)and the T.gondii B1 gene was amplified by nested PCR.In total,we analyzed 403 blood samples of black-headed gulls,128 of Rattus norvegicus,and 34 of free-range native chickens in the areas of Fuxian Lake and Dianchi Lake.The antibody posotive rate of T.gondii antibodies was 11.40%(44/386),and the detection rate of T.gondii B1 was 9.03%(51/565),including 3.23%(13/403)in black-headed gulls,17.19%(22/128)in Rattus norvegicus and 47.06%(16/34)in free-range native chickens.The results showed T.gondii infections in R.norvegicus and free-range native chickens in the habitat of black-headed gull.The survey provided preliminary information on the prevalence of T.gondii in wintering black-headed gulls and other sympatric species,which is of public health significance for the prevention and control of toxoplasmosis and a reference for the tracing,prevention and diagnosis of toxoplasmosis in migratory birds.
作者
董路
何紫薇
程文杰
杨建发
代飞燕
邹丰才
杨林富
DONG Lu;HE Ziwei;CHENG Wenjie;YANG Jianfa;DAI Feiyan;ZOU Fengcai;YANG Linfu(College of Veterinary Medicine,Yunnan Agricultural University,Kunming,650201,China)
出处
《野生动物学报》
北大核心
2021年第1期137-142,共6页
CHINESE JOURNAL OF WILDLIFE
基金
国家自然科学基金项目(31760730)
云南省专家工作站项目(202005AF150041)。
关键词
红嘴鸥
弓形虫
B1基因
Black-headed gulls
Toxoplasma gondii
B1 gene
作者简介
董路,男,25岁,硕士研究生,主要从事动物寄生虫病与寄生虫学研究。E-mail:1342428678@qq.com;通讯作者:杨林富,E-mail:1346279271@qq.com。
