摘要
利用组培技术,以凤头姜(Zingiber officinale Roscoe cv.Fengtoujiang)茎尖为外植体,采用不同激素组合,筛选最佳培养基配方,构建凤头姜脱毒快繁体系。结果表明,MS+6-BA(1.5 mg/L)+NAA(0.1 mg/L)培养基对愈伤组织及茎尖成活率的影响最佳;MS+6-BA(2.0 mg/L)+KT(0.1 mg/L)培养基诱导愈伤组织形成的丛生芽多,且粗壮。MS+6-BA(1.0 mg/L)+NAA(0.3 mg/L)培养基不仅对根的分化最有效,还能促进分蘖及成苗,大大提高了繁殖系数。
To screen out the optimal culture medium and build the virus-free rapid propagation system for Fengtoujiang,experiment was carried out with tissue culture technology,using stem apex of as explant and different phytohormone combination.The results indi⁃cated that the optimal effect on survival of the callus and stem apex was the combination of MS+6-BA(1.5 mg/L)+NAA(0.1 mg/L).The formation of callus was induced by the medium of MS+6-BA(2.0 mg/L)+KT(0.1 mg/L)and the robust adventitious bud was in⁃creased as well.Not only had the MS+6-BA(1.0 mg/L)+NAA(0.3 mg/L)the most efficient effect on ginger root differentiation,but al⁃so improved tillering and stand establishment,which increased the propagation significantly.
作者
叶兴枝
程群
张等宏
徐怡
陈巧玲
陈火云
YE Xing-zhi;CHENG Qun;ZHANG Deng-hong;XU Yi;CHEN Qiao-ling;CHEN Huo-yun(Southern Potato Research Center of China Enshi/Enshi Academy of Agricultural Sciences,Enshi 445000,Hubei,China)
出处
《湖北农业科学》
2020年第23期156-158,共3页
Hubei Agricultural Sciences
关键词
凤头姜
组织培养
快繁
Zingiber officinale Roscoe cv.Fengtoujiang
tissue culture
rapid propagation
作者简介
叶兴枝(1983-),女,湖北恩施人,助理研究员,主要从事马铃薯、甘薯育种及脱毒快繁研究,(电话)15997766509,(电子信箱)154076738@qq.com;通信作者:程群(1971-),女,湖北恩施人,高级农艺师,主要从事马铃薯、甘薯育种及脱毒快繁研究,(电话)18986846666,(电子信箱)quyongbreeding@163.com。
