摘要
为获得菌球密度高、直径小和活力强的广叶绣球菌液体菌种,经优化试验,得到适宜培养条件为培养基初始pH 5.0,装液量140~170 mL,接种量9%,摇床转速120~150 r/min;并确定液体菌种的适宜培养终点为9~10天,此时菌球直径1.07±0.03 mm,菌球密度111±11.53个/mL,接种后菌丝生长速度2.34±0.18 mm/d。
Liquid spawn could reduce the spawn running time and allows the antomation of inoculaton, which is crucial for industrial large scale production. In order to obtain liquid spawn of Sparassis latifolia with high density, small diameter and more vigorous mycelium pellet, the culture conditions and its terminal points were optimized. The optimal culture conditions for maximum biomass production were listed as follows: initial medium pH 4.0, liquid volume 140~170 mL, inoculation volume 9% and rotation speed 120~150 r/min. The terminal point of liquid spawn culture is 9~10 days after inoculation, under this conditon, pellet diameter is 1.07±0.03 mm, pellet density is 111±11.53 pellets/mL and mycelial growth rate is 2.34±0.18 mm/d. the results obtained in this work will be beneficial for commercial cultivation of Sparassis latifolia.
作者
马璐
杨驰
肖冬来
应正河
江晓凌
刘晓瑜
林衍铨
Ma Lu;Yang Chi;Xiao Donglai;Ying Zhenghe;Jiang Xiaoling;Liu Xiaoyu;Lin Yanquan(Institute of Edible Fungi,Fujian Academy of Agricultural Sciences,National and Local Joint Engineering Research Center for Breeding&Cultivation of Featured Edible Fungi,Fuzhou,Fujian 350014,China)
出处
《食药用菌》
2020年第6期428-433,共6页
Edible and Medicinal Mushrooms
基金
福建省属公益类科研院所基本科研专项(2018R1020-4、2018R1020-3)
福建省农业科学院科技创新团队项目(STIT2017-1-6)
福建省科技特派员后补助项目绣球菌工厂化栽培技术及产业化平台建设。
关键词
液体培养
培养条件
培养终点
菌丝活力
liquid culture
culture conditions
terminal point of cultivation
mycelial activity
作者简介
马璐(1984—),男,硕士,主要研究方向为食药用菌工厂化栽培。E-mail:malujj@163.com;通讯作者:林衍铨(1963—),男,研究员,主要从事食用菌育种与工厂化栽培的研究与开发,E-mail:lyq-406@163.com。
