摘要
研究江西南康3种柚子“水晶柚”、“练家柚”和“沙田柚”果皮精油的挥发性成分和含量差异,为南康甜柚品种改良及鉴定提供参考。以相似种植环境的3种柚子品种的果皮为材料,利用蒸馏法提取精油,使用顶空气相色谱质谱联用仪(headspace gas chromatography-mass spectrometer,HS-GC-MS)对精油成分进行鉴定和分析,并使用SIMCA14.0对试验数据进行预处理、主成分分析(principal component analysis,PCA)和正交偏最小二乘法-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)分析,结合使用SPSS22.0进行t检验和聚类分析。结果表明:3种柚子果皮精油成分和含量存在较大差异。3种柚子果皮精油共检测到49种物质;沙田柚果皮精油检出47种成分;水晶柚和练家柚有46种成分。化合物类型主要为烯类(11种)、醇类(13种)、酯类(9种)和醛类(5种)。通过t检验(P<0.05)和OPLS-DA分析发现,练家柚与沙田柚、水晶柚与练家柚均存在10种差异成分,而水晶柚与沙田柚存在8种差异成分,从3种柚子精油PCA分析和聚类分析上看,水晶柚划分为一类,练家柚划分一类,沙田柚划分一类。
The volatile constituents and content differences of peel oils from three types of grapefruit“Crystal pomelo”,“Lianjia pomelo”and“Shatian pomelo”in Jiangxi Nankang were studied to provide a reference for the improvement and identification of Nankang sweet pomelo varieties.Using the peels of three grapefruit varieties grown in the same habitat as materials,the essential oil was extracted by distillation,and the components of the essential oil were identified and analyzed using headspace gas chromatography-mass spectrometry(HS-GC-MS).The experimental data were pre-processed,principal component analysis(PCA)analysis and orthogonal partial least squares-discriminant analysis(OPLS-DA)analysis,combined with SPSS 22.0 for t test and cluster analysis.The results showed that there were significant differences in the composition and content of the essential oils of the three grapefruit peels.A total of 49 substances were detected in three types of grapefruit peel essential oils,47 components were detected in Shatian pomelo peel essential oils,and crystal grapefruit and Lianjia pomelo had 46 components.The main types of compounds were terpenes(11 species),alcohols(13 species),esters(9 species),and aldehydes(5 species).By t test(P<0.05)and OPLS-DA analysis,it was found that there were 10 different components in Lianjia pomelo and Shatian pomelo,Crystal pomelo and Lianjia pomelo,and 8 different components in Crystal pomelo and Shatian pomelo.From the perspective of PCA analysis and cluster analysis of grapefruit essential oil,Crystal grapefruit was divided into one category,Lianjia grapefruit was divided into one category,and Shatian grapefruit was divided into one category.
作者
李俭
钟八莲
姚锋先
刘桂东
刘璐
钟余
彭婷
LI Jian;ZHONG Ba-lian;YAO Feng-xian;LIU Gui-dong;LIU Lu;ZHONG Yu;PENG Ting(College of Life Science,Gannan Normal University,Ganzhou 341000,Jiangxi,China;National Navel Orange Engineering Technology Research Center,Ganzhou 341000,Jiangxi,China)
出处
《食品研究与开发》
CAS
北大核心
2020年第24期173-180,共8页
Food Research and Development
基金
国家自然科学基金(31760563)
江西省自然科学基金(20171BAB204024)。
关键词
主成分分析
顶空气相色谱-质谱法
差异成分
正交偏最小二乘法-判别分析
聚类分析
principal component analysis
headspace gas chromatography-mass spectrometry(HS-GC-MS)
differential components
orthogonal partial least squares-discriminant analysis(OPLS-D)
cluster analysis
作者简介
李俭(1994-),男(汉),硕士研究生,研究方向:柑桔遗传育种与生理;通信作者:彭婷,女,副教授,博士,研究方向:柑桔逆境生理及分子机理和遗传育种。
