摘要
目的研究分析采用稀释回归法检测脂血标本凝血酶原时间(PT)以及活化部分凝血活酶时间(APTT)的价值与可行性。方法抽选2019年1月—2020年1月间该院30例正常凝血标本(无溶血、脂血以及黄疸),检测原血浆PT以及APTT水平,另分别在3倍和5倍稀释状态下检测血浆PT及APTT水平,采用线性回归方法分析处理稀释前后的数据,计算相关指标回归方程;另将2019年1月—2020年1月来该院接受检查的90例门诊及住院患者纳入该次研究,根据三酰甘油浓度水平,将其分为轻度脂血组(30例)、中度脂血组(30例)和重度脂血组(30例),分别在高速离心处理和3倍稀释处理后检测3组患者血浆样本的PT以及APTT浓度值。以正常凝血标本检测结果计算所得回归方程为基础,录入3组脂血标本稀释后检测数据,将所得结果与高速离心法检测数据作对比分析。结果5倍稀释状态下,绝大多数正常凝血标本无法检出结果。3倍稀释后检测发现,稀释回归法检测轻、中度以及重度脂血标本的PT(10.673±1.549)s、(11.774±2.594)s、(17.006±6.834)s,APTT(29.925±10.228)s、(30.858±8.171)s、(33.203±9.897)s,与采用高速离心法检测相比,差异无统计学意义(t=0.367、0.817、0.560,P=0.715、0.417、0.578;t=1.522、0.905、1.042,P=0.134、0.369、0.302)。结论以高速离心法检测数据为参考,稀释回归法检测脂血标本凝血指标所得数据可靠,临床可视具体情况选择应用。
Objective To study the value and feasibility of detecting the prothrombin time(PT)and activated partial thromboplastin time(APTT)of lipemia samples by dilution regression method.Methods Selected 30 normal coagulation specimens(without hemolysis,lipemia,and jaundice)in the hospital from January 2019 to January 2020 to detect the levels of PT and APTT in the original plasma.In addition,the plasma PT and APTT levels were measured at 3 times and 5 times dilution respectively,and the data before and after the dilution were analyzed and processed by linear regression method,and the regression equation of related indexes was calculated;in addition,90 outpatient and inpatient patients who came to the hospital for examination from January 2019 to January 2020 were included in this study.According to the level of triacylglycerol concentration,they were divided into mild lipemia group(30 cases),moderate lipemia group(30 cases)and severe lipemia group(30 cases).They were treated by high-speed centrifugation and 3 times dilution treatment respectively.Afterwards,the PT and APTT concentration values of the plasma samples of the three groups of patients were detected.Based on the regression equation calculated from the test results of normal coagulation specimens,the test data of three groups of lipemia specimens after dilution were entered,and the results were compared with the test data of high-speed centrifugation.Results In the 5-fold dilution state,most normal coagulation specimens could not detect the results.After 3-fold dilution,it was found that the dilution regression method detected PT(10.673±1.549)s,(11.774±2.594)s,(17.006±6.834)s,APTT(29.925±10.228)s of light,moderate and severe lipemia samples,(30.858±8.171)s,(33.203±9.897)s,compared with the high-speed centrifugation method,the difference was not statistically significant(t=0.367,0.817,0.560,P=0.715,0.417,0.578;t=1.522,0.905,1.042,P=0.134,0.369,0.302).Conclusion The data obtained by the high-speed centrifugation method is used as a reference,and the data obtained by the dilution regression method for the detection of coagulation indexes of lipid samples are reliable,and the clinical application can be selected according to the specific situation.
作者
王静
WANG Jing(Department of Laboratory Medicine,Sihong County People's Hospital,Suqian,Jiangsu Province,223900China)
出处
《系统医学》
2020年第22期34-36,共3页
Systems Medicine
关键词
脂血标本
稀释回归法
凝血酶原时间
凝血活酶时间
Lipid specimen
Dilution regression method
Prothrombin time
Thromboplastin time
作者简介
王静(1976-),女,本科,副主任技师,主要从事急诊检验工作。
