摘要
目的分析长链非编码RNA(long non-coding RNA,lncRNA)淋巴细胞白血病缺失基因1(deleted in lymphocytic leukemia 1,DLEU1)在食管鳞癌(esophageal squamous cell carcinoma,ESCC)中的表达及意义,并进一步研究DLEU1对ESCC细胞TE-13增殖、迁移和侵袭的影响。方法采用实时荧光定量PCR检测ESCC组织和癌旁组织中DLEU1的表达。通过CCK-8、划痕试验和Transwell侵袭试验分析DLEU1表达对TE-13增殖、迁移和侵袭的影响。结果DLEU1在ESCC癌组织中的表达较癌旁组织明显升高(P<0.05)。DLEU1的表达与淋巴结转移和TNM分期相关(P<0.05),而与患者的年龄、性别、分化程度和浸润深度均无关(P>0.05)。生存分析示,DLEU1高表达ESCC患者的生存时间较低表达患者明显缩短(P<0.05)。干扰DLEU1表达后TE-13的增殖、迁移和侵袭能力显著受抑。结论DLEU1在ESCC中表达上调,DLEU1的高表达提示预后不良,DLEU1可能对ESCC细胞TE-13的增殖、迁移和侵袭具有调节作用,DLEU1有可能成为ESCC治疗的潜在靶点。
Objective To analyze the expression of long-chain non-coding RNA(lncRNA)lymphocyte leukemia deletion gene 1(DLEU1)in esophageal squamous cell carcinoma(ESCC)and its clinical significance.The effect of DLEU1 on the proliferation,migration and invasion of TE-13 was further studied.Methods Real-time quantitative polymerase chain reaction was used to detect the expression of DLEU1 in ESCC and adjacent normal tissues.The effects of DLEU1 on the proliferation,migration and invasion of TE-13 were analyzed by CCK-8,scratch test and Transwell test.Results The expression of DLEU1 in ESCC was significantly higher than that in adjacent normal tissues(P<0.05).The expression of DLEU1 was correlated with lymph node metastasis and TNM stage(P<0.05),but not with age,gender,degree of differentiation and depth of invasion(P>0.05).After knockdown of DLEU1 in TE-13 cells,cell proliferation,migration and invasion were significantly suppressed.Conclusion The expression of DLEU1 was up-regulated in ESCC,and the high expression of DLEU1 suggested a poor prognosis.DLEU1 may have a regulatory effect on the proliferation,migration and invasion of TE-13 cells,and it may become a potential target for ESCC treatment.
作者
顾术东
缪捷飞
沈洪
张曙
茅国新
GU Shudong;MIAO Jiefei;SHEN Hong;ZHANG Shu;MAO Guoxin(Department of Oncology,Affiliated Hospital of Nantong University,Nantong 226001,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2020年第21期2911-2915,共5页
The Journal of Practical Medicine
基金
南通市科技局项目(编号:JC2018154)。
关键词
淋巴细胞白血病缺失基因1
食管鳞癌
增殖
迁移
侵袭
deleted in lymphocytic leukemia 1
esophageal squamous cell carcinoma
proliferation
migration
invasion
作者简介
通信作者:茅国新,E-mail:Maogx333@126.com。
