摘要
目的:探讨对比抗酸染色与实时荧光定量PCR法检测在肺结核辅助诊断中的应用效果。方法:选择本院接受研究的患者100例,将其送检标本进行石蜡包埋组织切片处理,并在镜下形态学的基础上对组织进行观察,得出其均表现为肉芽肿性炎伴随坏死问题,并在后期进行抗酸染色和抽提组织核酸RT-PCR检测,对两种方法检测结果做记录分析。结果:抗酸染色法阳性率比RT-PCR法低,两种检测方法数据差异较大,存在统计学意义(P<0.05),并且RT-PCR法辅助诊断结核病的确诊率更高。结论:结核病进行病理学检测同时使用RT-PCT技术,其对应的检测灵敏度较高,在结核病辅助诊断中具有积极意义。
Objective:To investigate the application effect of comparing acid-fast staining and real-time fluorescent quantitative PCR in the auxiliary diagnosis of tuberculosis.Methods:Select 100 patients in our hospital for research,send their specimens for paraffin-embedded tissue section processing,and observe the tissues on the basis of microscopic morphology,and conclude that all of them are accompanied by granulomatous inflammation Necrosis,and acid-fast staining and RT-PCR detection of tissue nucleic acid extraction in the later stage,and record and analyze the detection results of the two methods.Results:The positive rate of acid-fast staining method was lower than that of RT-PCR method,and the data of the two detection methods were quite different and statistically significant(P<0.05),and the diagnosis rate of RT-PCR method for assisted diagnosis of tuberculosis was higher.Conclusion:The RT-PCT technique is also used for pathological detection of tuberculosis,and its corresponding detection sensitivity is relatively high.It has positive significance in the auxiliary diagnosis of tuberculosis and can be promoted and used in clinical practice.
作者
步晓琳
李宝华
连爱琼
陈惠芹
BU Xiao-lin;LI Bao-hua;LIAN Ai-qiong;CHEN Hui-qin(Department of Pathology,Xinglin Branch,the First Affiliated Hospital of Xiamen University,Fujian Xiamen 361021)
出处
《中国医疗器械信息》
2020年第22期25-26,94,共3页
China Medical Device Information
关键词
抗酸染色
实时荧光定量PCR法
肺结核
辅助诊断
对比
acid-fast staining
real-time fluorescence quantitative PCR
tuberculosis
auxiliary diagnosis
comparison
作者简介
通讯作者:步晓琳。
