摘要
【目的】明确海南省番茄晚疫病菌对甲霜灵的敏感性,筛选对该病原菌具有较高毒力和较好防治效果的杀菌剂。【方法】采用叶盘漂浮法测定132株菌株对甲霜灵的敏感性,采用离体叶片法测定10种原药及16种制剂对番茄晚疫病菌的抑菌活性,并测定9种制剂对番茄晚疫病的田间防治效果。【结果】对甲霜灵抗性型、中间型和敏感型的菌株分别占0、95.5%和4.5%,原药甲霜灵、嘧菌酯和烯酰吗啉以及制剂440 g/L精甲霜灵·百菌清SC、64%噁霜灵·代森锰锌WP和23.4%双炔酰菌胺SC对番茄晚疫病菌均有较高的抑菌活性,440 g/L精甲霜灵·百菌清SC和64%噁霜灵·代森锰锌WP对番茄晚疫病田间防效较好。【结论】海南省采集的132株菌株中以中间型占优势,甲霜灵、烯酰吗啉和嘧菌酯对海南省番茄晚疫病菌具有较高的抑菌活性,甲霜灵、噁霜灵、双炔酰菌胺和烯酰吗啉制剂是防治番茄晚疫病的有效药剂。
[Purposes]To clarify the sensitivity to metalaxyl of Phytophthora infestans isolates collected from Hainan Province and screen fungicides which have highly inhibitory activities and have high efficacy against P.infestans.[Methods]The sensitivity of 132 isolates to metalaxyl was determined by leaf disc floating test,the inhibitory activity of 10 originals and 16 formulations of fungicides against P.infestans was determined on the detached leaves,and the efficacy of 9 fungicide formulations in controlling tomato late blight was determined in the field.[Results]The metalaxyl-resistant,intermediate and sensitivity isolates were 0,95.5%and 4.5%,respectively.Metalaxyl,azoxystrobin,dimethomorph and metalaxyl-M·chlorothalonil 440 g/L SC,oxadixyl·mancozeb 64%WP,mandipropamid 23.4%SC had good inhibitory activity against P.infestans on the detached leaves of tomato.Metalaxyl-M·chlorothalonil 440 g/L SC and oxadixyl·mancozeb 64%WP had better control effect to tomato late blight in the field.[Conclusions]Intermediate isolates prevailed in the 132 isolates from Hainan Province,metalaxyl,dimethomorph and azoxystrobin have high inhibitory activity against P.infestans on detached tomato leaves.Formulations of metalaxyl,oxadixyl,mandipropamid and dimethomorph can be used to control tomato late blight.
作者
王红刚
陈银华
于晓惠
杨石有
陈雨娟
黄思源
王文桥
WANG Honggang;CHEN Yinhua;YU Xiaohui;YANG Shiyou;CHEN Yujuan;HUANG Siyuan;WANG Wenqiao(College of Tropical Crops,Hainan University,Haikou 570228,China;College of Forestry,Hainan University,Haikou 570228,China;Institute of Plant Protection,Hebei Academy of Agricultural and Forestry Sciences,IPM Center of Hebei Province,Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China,Ministry of Agriculture,Baoding 071000,China)
出处
《云南农业大学学报(自然科学版)》
CSCD
北大核心
2020年第6期957-962,982,共7页
Journal of Yunnan Agricultural University:Natural Science
基金
海南省自然科学基金项目(318MS017)
国家重点研发计划项目(2016YFD0201000)。
关键词
抗药性
番茄晚疫病菌
杀菌剂
抑制活性
田间药效
fungicide resistance
Phytophthora infestans
fungicide
inhibitory activity
control efficacy in field
作者简介
王红刚(1982-),男,河北磁县人,硕士,讲师,主要从事植物病害防治及杀菌剂应用技术研究。E-mail:deru666@163.com;通信作者:王文桥(1963-),男,湖北洪湖人,博士,研究员,主要从事植物病原菌抗药性及杀菌剂应用技术研究。E-mail:wenqiaow@163.com。