摘要
基于全基因组序列已经公布的黄单胞菌Xanthomonas campestris B100、Xanthomonas campestris pv.campestris 8004、Xanthomonas campestris CN14中分泌蛋白序列为基础数据,采用Protscale、SMART、TargetP 2.0 Server等生物信息学分析软件对上述分泌蛋白展开理化性质、保守结构域以及转运肽等分析,结果显示:黄单胞菌中分泌蛋白理论等电点与氨基酸长度之间无相关关系,44.73%的蛋白为不稳定蛋白,83.21%的蛋白亲水性小于0,属于亲水性蛋白。同时,在每种黄单胞菌的分泌蛋白中,具有明显保守结构域的蛋白数量平均为12个,并且所有分泌蛋白均具有信号肽,转运肽预测可能性分布也较为平均。
Xanthomonas campestris belong to Gram-negative bacteria,which mainly damage cruciferous vegetables and economic forests including walnuts and mangosteens,causing great economic losses to agricultural and forestry crops and seriously harming the healthy development of the agricultural and forestry industry.Secreted proteins play an important role in the pathogenic process of plant pathogenic fungi,bacteria,and oomycetes.There is no reports on the physicochemical properties and characteristics of secretary proteins of Xanthomonas.This study uses the secretary protein sequences of X.campestris B100,X.campestris pv.campestris str.8004,and X.campestris CN14 from the published genome-wide sequence as the basic data,and uses Protscale,SMART,TargetP 2.0 Server,and other bioinformatics software to analyze the physicochemical properties,conserved domains,and transit peptides of the secretary proteins above-mentioned.The results showed that there was no obvious rule between the theoretical isoelectric point of secretary proteins and the length of amino acids in X.campestris.An average 44.73%of the protein is unstable protein.An average of 83.21%of the protein has a total average hydrophilicity of less than 0,which is a hydrophilic protein.The number of proteins with obvious conserved domains in each secretary protein of Xanthomonas is 12 on average.All secretary proteins have signal peptides.The distribution of transit peptide prediction possibilities is relatively even.
作者
覃悦
祝友朋
韩长志
QIN Yue;ZHU Youpeng;HAN Changzhi(Yunnan Province Key Laboratory of Forest Disaster Warning and Control,College of Biodiversity Conservation,Southwest Forestry University,Kunming 650224,China)
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2020年第6期30-36,共7页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(31960314)
云南省应用基础研究计划项目(2018FG001-028)
云南省研发经费投入补助项目(2016YB462&2017YB236)。
关键词
黄单胞菌
分泌蛋白
理化性质
生物信息学分析
植物病原菌
亚细胞定位
转运肽
Xanthomonas
secretary protein
physicochemical properties
bioinformatics
pathogen
subcellular localization
transit peptide
作者简介
覃悦,硕士研究生.研究方向:资源利用与植物保护.E-mail:453276403@qq.com;通讯作者:韩长志,博士,副教授.研究方向:经济林木病害生物防治与真菌分子生物学.E-mail:hanchangzhi2010@163.com。
