摘要
为探究体色变异大鳞副泥鳅的胚胎发育特点,在(26.0±1.0)℃水温下,使用绒毛膜促性腺激素、促黄体素释放激素A2和地欧酮对体质量(56.92±3.23)g的大鳞副泥鳅选育品系“赣红1号”亲本人工催产,经过14.7 h的效应时间,采用半干法人工授精,观察并记录其胚胎各时期的发育过程和形态特征。试验结果显示,“赣红1号”平均催产率93.4%,受精率76.7%,孵化率72.3%;受精卵为沉性卵,具黏性,受精卵吸水膨胀后,平均膜径为1052.49μm;受精卵在水温(26.9±0.8)℃、溶解氧≥8.25 mg/L、pH 8.2的条件下,经历胚盘形成、卵裂、囊胚、原肠、神经胚、器官形成和出膜7个阶段29个发育时期,历时26.42 h开始孵化出膜,胚胎发育总积温为713.25℃·h。初孵仔鱼无黑色素,平均全长3193.42μm,肌节40节以上,心搏率72~85次/min,多沉在水底,偶作不规则短距离冲游。试验结果表明,“赣红1号”胚胎发育时序与正常大鳞副泥鳅相同,两者在出膜期表现出体色差异。
A new strain of bigscale loach Paramisgurnus dabryan,“Ganhong No.1”,with body weight of(56.92±3.23)g were induced to spawn by injection of mixture of luteinizing hormone releasing hormone A2,domperidone and chorionic gonadotropin at water temperature of(26.0±1.0)℃.After the eggs were fertilized in 14.7 h by semi-dry artificial insemination,the developmental phases and morphological characteristics of embryo were observed and described in order to understand the embryonic development characteristics of variation of body color of bigscale loach“Ganhong No.1”strain.It was found that the“Ganhong No.1”had success rate of 93.4%,fertilization rate of 76.7%,and hatching rate of 72.3%.The fertilized eggs were demersal and adhensive,expansion after water absorption,with average diameter of 1052.49μm.The fertilized eggs were hatched via 7 stages including blastoderm,cleavage,blastula,gastrula,neuroblasts,organogenesis and hatching under the conditions of water temperature of(26.9±0.8)℃,dissolved oxygen level≥8.25 mg/L,and pH 8.2,with total accumulative temperature of 713.25℃·h.Newly hatched larvae with an average total length of 3193.42μm had no melanin,and submergenced to the bottom,and occasionally making irregular short-distance rushes,with more than 40 myomeres,and heart beating rate of 72 to 85 beats/min.The findings indicated that the timing of embryo development of“Ganhong No.1”was the same as that of the normal bigscale loach,with difference in body color in both loach types during the hatching stage.
作者
马本贺
陶志英
吴早保
吴斌
王海华
徐先栋
陈翠云
MA Benhe;TAO Zhiying;WU Zaobao;WU Bin;WANG Haihua;XU Xiandong;CHEN Cuiyun(Jiangxi Fisheries Research Institute, Nanchang 330039, China;Fisheries Bureau of Yingtan City, Yingtan 335000, China;Yujiang Hongxin Special Aquaculture Limited Corporation, Yingtan 335200, China)
出处
《水产科学》
CAS
CSCD
北大核心
2020年第6期863-870,共8页
Fisheries Science
基金
江西省现代农业产业技术体系建设专项(JXARS-10)
鹰潭市重点研发计划项目(NY201823).
关键词
大鳞副泥鳅
赣红1号
人工繁殖
胚胎发育
Paramisgurnus dabryanus
Ganhong No.1
artificial propagation
embryonic development
作者简介
马本贺(1990—),男,助理研究员,研究方向:水产动物健康养殖和遗传育种.E-mail:mabenhe@126.com;通讯作者:王海华(1971—),男,研究员,博士,研究方向:渔业资源和水产养殖.E-mail:haihuawang998@sina.com.cn.
