摘要
目的目前对艾滋病病毒(HIV)血浆控制者机体免疫机制的研究仍然较少。文中旨在分析HIV血浆控制者外周血淋巴细胞亚群的分布及其相关性,了解其免疫功能状况,为研究其免疫机制提供参考。方法选取江苏省2016年1月至2018年12月29例HIV-1抗体确证5年以上的阳性患者。根据患者CD4细胞计数和病毒载量水平分为:血浆控制组[病毒载量<2000 copies/mL,平均病毒载量(991±887)copies/mL,n=6]、慢性感染组[病毒载量≥2000 copies/mL,平均病毒载量(52181±10180)copies/mL,n=23],另选取健康人群为对照组(n=70)。采用流式细胞仪对各组人群外周血淋巴细胞亚群进行检测,比较3组间CD4+ T淋巴细胞、CD8+ T淋巴细胞、NK细胞和B细胞的绝对计数,分析各组外周血淋巴细胞亚群的相关性。结果血浆控制组、慢性感染组CD4+T、CD8+T计数明显低于健康对照组(P<0.05),血浆控制组CD4+T计数明显高于慢性感染者组[(697.9±160.4)个/μL vs(399.3±118.7)个/μL,P<0.05]。慢性感染组NK细胞计数明显低于健康对照(P<0.05),血浆控制者组和健康对照组差异无统计学意义(P>0.05)。血浆控制组与健康对照组B细胞计数[(322.0±261.5)个/μL、(294.9±86.2)个/μL]明显高于慢性感染组[(127.1±98.7)个/μL],差异有统计学意义(P<0.05)。血浆控制组外周血CD4+、B和NK细胞计数之间两两呈正相关(P<0.05)。结论血浆控制者各淋巴细胞细胞亚群的数量高于慢性感染者,淋巴细胞亚群之间存在积极的相互促进关系,为进一步研究血浆控制者免疫作用机制提供了参考。
Objective At present,there are still few researches on the immune mechanism of HIV-1 viremic controllers.This paper aims to analyze the distribution and correlation of peripheral blood lymphocyte subsets in HIV-1 viremic controllers,to understand their immune function status,and to lay a foundation for the study of their immune mechanism.Methods Twenty-nine patients confirmed positive for HIV-1 antibody in Jiangsu Provincial from January 2016 to December 2018 were chosen.According to their CD4 cell count and viral load level,patients were divided into:plasma control group[viral load<2000copies/mL,average viral load(991±887 copies/mL,n=6]and chronic infection group[viral load^2000 copies/mL,average viral load(52,181±10180 copies/mL,n=23].In addition,the healthy people in the centre were selected as the control group(n=10).Using flow cytometry to test the peripheral blood lymphocyte subsets in each group,we compared the absolute count of CD4^+T lymphocytes and CD8^+T lymphocytes,NK cells and B cells among three groups,analyzed the correlation of peripheral blood lymphocyte subsets.Results CD4^+T and CD8^+T counts in plasma control group and chronic infection group were significantly lower than those in control group(P<0.05),and CD4^+T counts in plasma control group were significantly higher than that in chronic infection group[(697.9±160.4)vs(399.3±118.7)/L,P<0.05)].NK cell count in the chronic infection group was significantly lower than that in control group(P<0.05),and the difference between the plasma control group and the control group was not statistically significant(P>0.05).The number of B cells[(322.0±261.5)/L,(294.9±86.2)/L]in plasma control group and control group was significantly higher than that in chronic infection group[(127.1±98.7)/L],and the difference was statistically significant(P<0.05).The count of CD4^+cells,B cells and NK cells in peripheral blood of the plasma control group was positively correlated with each other(P<0.05).Conclusion The number of lymphocyte subsets in plasma control patients was higher than that in chronic infection patients,and the lymphocyte subsets were positively correlated with each other,which provided a reference for further study on immune mechanism of viremic control patients.
作者
张之
胡海洋
葛尤
卢静
周莹
徐晓琴
邱涛
傅更锋
ZHANG Zhi;HU Hai-yang;GE You;LU Jing;ZHOU Ying;XU Xiao-qin;QIU Tao;FU Geng-feng(Department of HIV/STD Control and Prevention,Jiangsu Provincial Center for Disease Control and Prevention,Nanjing 22500,Jiangsu,China;Department of Epidemiology and Health Statistics,School of Public Health,Southeast University,Nanjing 225QQ,Jiangsu,China)
出处
《医学研究生学报》
CAS
北大核心
2020年第10期1051-1055,共5页
Journal of Medical Postgraduates
基金
国家科技重大专项(民口)课题(2018ZX10715-002)
江苏省卫生健康委科研课题(Z2018039)。
作者简介
通信作者:傅更锋,E-mail:fugf@jscdc.cn。
