摘要
苦荞中的黄酮类化合物具有很高的药用价值和保健功能。以苦荞川荞1号为材料,克隆出FtF3GT1基因,其CDS全长为1 401bp,编码467个氨基酸。系统发育分析表明,FtF3GT1与甜荞中的FeUF7GT蛋白亲缘关系最近。组织特异性表达分析结果表明,FtF3GT1基因在各个组织中均有表达,在根部最低,在茎部最高;MeJA处理苦荞和转pCAMBIA3301:FtF3GT1pro::GUS毛状根中发现,FtF3GT1基因的表达量以及GUS的活性明显受MeJA诱导;转过表达FtF3GT1基因毛状根中,总黄酮含量明显升高,推测FtF3GT1基因可促进黄酮类物质的合成。
Flavonoids in tartary buckwheat have high medicinal value and health function. Based on the tartary buckwheat Chuanqiao No.1, FtF3GT1 gene was cloned from tartary buckwheat and its CDS length was 1 401 bp, encoding 467 amino acids. Phylogenetic analysis showed that FtF3GT1 was closely related to FeUF7GT protein in common tartary buckwheat. Specific expression analysis results showed that FtF3GT1 gene was expressed in all tissues, but the lowest in roots and the highest in stems. Treated with MeJA, expression of FtF3GT1 gene and GUS activity in pCAMBIA3301:FtF3GT1 pro::GUS in transgenic hairy roots of tartary buckwheat were significantly induced by MeJA, it is speculated that FtF3GT1 gene was induced by MeJA and promoted the biosynthesis of flavonoids synthesis.
作者
卢晓玲
何铭
张凯旋
廖志勇
周美亮
Lu Xiaoling;He Ming;Zhang Kaixuan;Liao Zhiyong;Zhou Meiliang(College of Life and Environmental Science,Wenzhou University,Wenzhou 325035,Zhejiang,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《作物杂志》
CAS
北大核心
2020年第5期33-40,共8页
Crops
基金
国家重点研发计划中国-欧盟国际合作重点项目(2017YFE0117600)
国家自然科学基金面上项目(31871536)。
关键词
克隆
苦荞
黄酮合成
Cloning
Tartary buckwheat
Flavonoids synthesis
作者简介
卢晓玲,主要从事荞麦品质代谢调控方面研究,E-mail:luxiaoling07@163.com;通信作者:廖志勇,主要从事药用动植物活性化合物的分离、改造与活性研究,E-mail:zyliao@wzu.edu.cn;通信作者:周美亮,主要从事荞麦种质资源与品质代谢调控研究,E-mail:zhoumeiliang@caas.cn。
