摘要
[目的]建立了液相色谱-三重四极杆质谱测定枸杞中甲基硫菌灵及其代谢物多菌灵的检测方法。[方法]枸杞样品用0.1%甲酸乙腈提取,上清液经C18净化填料净化后,用液相色谱-质谱检测,外标法定量。[结果]甲基硫菌灵加标回收率:枸杞鲜果为82.3%~105.1%,相对标准偏差为1.8%~3.5%;枸杞干果为98.7%~104.1%,相对标准偏差为1.8%~5.4%。多菌灵的加标回收率:枸杞鲜果为91.4%~106.2%,相对标准偏差为2.2%~4.1%;枸杞干果为88.4%~101.9%,相对标准偏差为1.1%~3.1%。[结论]该方法的回收率和精密度均较好,为甲基硫菌灵在枸杞上的限量制标提供参考依据。
[Aims]This study aims to establish a novel method based on high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-MS/MS)for determination of thiocarbendazim-methyl and its metabolite carbendazim in Lycium.[Methods]The samples of Lycium fruit were extracted with 0.1%acetonitrile formate,purified with C18,and then detected by HPLC-MS/MS.[Results]The results showed that the average recoveries of thiocarbendazim-methyl residues in fresh Lycium raged from 82.3 to 105.1%with RSD of 1.8-3.5%.The average recoveries of thiocarbendazim-methyl residues in dry Lycium raged from 98.7 to 104.1%with RSD of 1.8-5.4%.The average recoveries of carbendazim residues in fresh Lycium raged from 91.4 to 106.2%with RSD of 2.2-4.1%.The average recoveries of carbendazim residues in dry Lycium raged from 88.4 to 101.9%with RSD of 1.1-3.1%.[Conclusions]The recovery rate and precision of the methods are good,which provide reference basis for the limitation of Lycium.
作者
杨静
吴燕
牛艳
赵子丹
刘霞
王晓菁
YANG Jing;WU Yan;NIU Yan;ZHAO Zi-dan;LIU Xia;WANG Xiao-jing(Quality Standards and Testing Institute of Agricultural Technology,Yinchuan 750002,China)
出处
《农药》
CAS
CSCD
北大核心
2020年第10期739-742,共4页
Agrochemicals
基金
宁夏枸杞质量安全评价及标准研究(QCYL-2018-05)
农业行业标准制修订项目(181821301092372)。
关键词
枸杞
甲基硫菌灵
多菌灵
检测
Lycium
thiocarbendazim-methyl
carbendazim
detection
作者简介
杨静(1986-),女,四川射洪人,助理研究员,主要从事农产品农药残留检测方法的研究。E-mail:289184175@qq.com;通讯作者:王晓菁(1972-),女,宁夏固原人,研究员,硕士,主要从事农产品质量标准相关研究。E-mail:niuyan98@163.com。
