摘要
以杨梅叶为原料,茶皂素为提取强化剂,采用超声波辅助提取工艺,在单因素试验的基础上通过正交试验优化了杨梅素的提取工艺,并考察了杨梅素的抗氧化活性。研究结果表明:杨梅素提取的最佳工艺条件为液料比15∶1(mL∶g),乙醇体积分数80%,提取温度70℃,提取时间50 min,表面活性剂茶皂素用量为原料质量的0.3%,在此条件下提取得率最高可达1.33%。当质量浓度为3.0 mg/L时,杨梅素对DPPH·的清除率最高可达到52.11%,IC 50值为2.670 mg/L,此时V C的清除率为56.49%,IC 50值为2.363 mg/L;质量浓度为120 mg/L的杨梅素对·OH清除率最高达到64.21%,此时V C的清除率为72.11%,杨梅素和V C对·OH的IC 50值分别为91.139和61.483 mg/L;可以看出,杨梅素具有较好的抗氧化能力,但整体效果略低于V C。
Using Myrica rubra leaves as raw materials and tea saponin as extraction enhancer,the ultrasonic-assisted extraction process was used to optimize the extraction process of myricetin by orthogonal test on the basis of single factor test,and the antioxidant activity of myricetin was investigated.The results showed that the best processing conditions for the extraction of myricetin were liquid-to-material ratio 15∶1(mL∶g),ethanol volume fraction 80%,extraction temperature 70℃,extraction time 50 min,and the amount of surfactant tea saponin 0.3%.Under these conditions,the extraction yield could reach up to 1.33%.When the mass concentration was 3.0 mg/L,the scavenging rate of myricetin to DPPH·could reach 52.11%and the IC 50 value was 2.670 mg/L;the scavenging rate of V C to DPPH·was 56.49%and the IC 50 value was 2.363 mg/L.The·OH scavenging rate of myricetin at the mass concentration of 120 mg/L reached the maximum of 64.21%,and the V C scavenging rate was 72.11%,and the IC 50 values were 91.139 and 61.483 mg/L,respectively;it could be seen that myricetin had a better anti-oxidation ability,but the overall effect was slightly lower than V C.
作者
童天娇
刘军海
刘艳
曹瑞
魏玲玲
TONG Tianjiao;LIU Junhai;LIU Yan;CAO Rui;WEI Lingling(College of Chemistry&Environmental Science,Shaanxi University of Technology,Hanzhong 723000,China)
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2020年第5期114-120,共7页
Chemistry and Industry of Forest Products
关键词
杨梅素
表面活性剂
超声波辅助提取
生物活性
myricetin
surfactant
ultrasonic-assisted extraction
biological activity
作者简介
童天娇(1995-),女,甘肃天水人,硕士生,研究方向为天然产物提取;通讯作者:刘军海,副教授,硕士生导师,研究领域为天然产物提取与深加工,E-mail:iamliujunhai@126.com。
