摘要
为初步了解新疆哈密市牛羊口蹄疫病毒3ABC非结构蛋白抗体情况,利用单抗阻断ELISA试验对来自哈密市3个区(县)未进行口蹄疫疫苗免疫的755份牛羊血清进行了检测。结果表明:伊吾县3ABC抗体阳性率显著高于伊州区(P<0.05),伊吾县3ABC抗体阳性率高于巴里坤县(P>0.05),差异不显著。不同饲养方式间抗体阳性率亦存在差异,牧区阳性率(17.03%)、半牧区(16.36%)显著高于农区(6.84%)(P<0.05);不同品种间,哈密本地羊(哈萨克羊)3ABC抗体阳性率为18.6%,显著高于山羊的3ABC抗体阳性率8.16%、多浪羊3ABC抗体阳性率为6.06%(P<0.05);不同性别间,母畜阳性率高于公畜;不同畜龄间,3ABC抗体阳性率与年龄显著相关(P<0.05);实验结果说明,哈密市牲畜存在口蹄疫自然感染的情况,要根除和消灭本病,需进一步加强3ABC抗体监测、引种检疫以及阳性牲畜的隔离、淘汰工作。
For the purpose of preliminarily understanding the antibody level of non-structural protein 3ABC of foot-and-mouth disease(FMD)in Hami city,755 serum samples of cattle and sheep from three districts(counties)of Hami city who were not immunized with FMDV vaccine were detected by McAb blocking ELISA.The results showed that the positive rate of 3ABC antibody in Yiwu county was significantly higher than that in Yizhou district(P<0.05),and the positive rate of 3ABC antibody in Yiwu county was higher than that in Balikun county(P>0.05),the difference was not significant.The positive rates of antibodies were also different among different feeding methods,the positive rate in pastoral area(17.03%)and semi-pastoral area(16.36%)were significantly higher than that in agricultural area(6.84%)(P<0.05);for different breeds of animals,the positive rate of 3ABC antibody of Hami local sheep(Kazak sheep)was 18.6%,which was significantly higher than that of goat(8.16%)and Duolang sheep(6.06%)(P<0.05);the positive rate of female livestock was higher than that of male livestock;the positive rate of 3ABC antibody was significantly correlated with age among different breeds(P<0.05).As a conclusion,there was a natural infection of FMD in Hami city.To eradicate and eliminate the disease,it is necessary to further strengthen the monitoring of 3ABC antibody,intensify the quarantine for introduced livestock,and to strengthen the isolation and elimination of positive livestock.
作者
关团
古丽巴哈尔·卡德尔
买吾兰·孜克牙
阿达来提·尤素甫
马春江
GUAN Tuan;GURIBAHAR·Kader;MAIWLAN·Zikya;ADALATI·Yousuf;MA Chun-jiang(Hami Animal Disease Prevention and Control Center,Hami 839000,China)
出处
《草食家畜》
2020年第5期46-51,共6页
Grass-Feeding Livestock
基金
哈密市人畜共患病畜间防控技术与应用(2018E02002)。
关键词
口蹄疫
非结构蛋白3ABC抗体
哈密市
foot-and-mouth disease
antibody against non-structural protein 3ABC
Hami city
作者简介
关团(1988-),男,硕士研究生,兽医师,研究方向为动物疫病防控。E-mail:496313288@qq.com;通讯作者:马春江(1972-),男,本科,农业技术推广研究员,研究方向为动物疫病防控。E-mail:1580109196@qq.com。
