摘要
目的探讨核苷酸结合寡聚化结构域样受体蛋白12(nucleotide binding oligomerization domain like receptor protein 12,NLRP12)在糖尿病并发冠心病患者中的作用。方法选取2018年6月至2019年10月烟台市烟台山医院内分泌科收治的单纯2型糖尿病(type 2 diabetes mellitus,T2DM)患者40例为单纯糖尿病组,冠心病患者80例为单纯冠心病组,T2DM合并冠心病患者80例为糖尿病并发冠心病组,同期体检的健康人60例为对照组。分离并培养外周血单核细胞,RT-PCR和Western blot检测NLRP12、凋亡相关斑点样蛋白、Caspase-3mRNA和蛋白表达水平。分离血浆,酶联免疫吸附法检测IL-18含量。结果单纯糖尿病组、单纯冠心病组、糖尿病并发冠心病组患者外周血单核细胞中NLRP12、凋亡相关斑点样蛋白、Caspase-3 mRNA和蛋白相对表达量明显高于对照组,差异有统计学意义(P<0.05);糖尿病并发冠心病组患者外周血单核细胞中凋亡相关斑点样蛋白、Caspase-3 mRNA和蛋白相对表达量明显高于单纯糖尿病组、单纯冠心病组,差异有统计学意义(P<0.05);NLRP12 mRNA和蛋白相对表达量在单纯糖尿病组、单纯冠心病组、糖尿病并发冠心病组中差异无统计学意义(P>0.05)。单纯糖尿病组、单纯冠心病组、糖尿病并发冠心病组血浆IL-18水平明显高于对照组,差异有统计学意义(P<0.01);糖尿病并发冠心病组血浆IL-18水平明显高于单纯糖尿病组、单纯冠心病组,差异有统计学意义(P<0.05)。相关性分析显示,血浆IL-18水平与NLRP12、凋亡相关斑点样蛋白、Caspase-3mRNA和蛋白相对表达量均呈正相关。结论NLRP12异常激活通过活化凋亡相关斑点样蛋白和Caspase-3表达,促进其下游炎症因子IL-18的成熟与释放,参与糖尿病并发冠心病的发生与发展。
Objective To investigate the role of nucleotide-binding oligomerization domain-like receptor protein 12(NLRP12)in diabetic patients with coronary heart disease.Methods From Jun.2017 to Oct.2018,40 patients with type 2 diabetes mellitus(T2DM)in our hospital were selected as simple diabetes group,80 patients with coronary heart disease as simple coronary heart disease group,80 patients with T2DM and coronary heart disease as diabetes mellitus complicated with coronary heart disease group,and 60 healthy people in the same period as control group.Peripheral blood mononuclear cells were isolated and cultured.The levels of NLRP12,apoptosis-related spot-like protein,Caspase-3 mRNA and protein expression were detected by RT-PCR and Western blot.The plasma was separated and the content of IL-18 was detected by ELISA.Results The relative expressions of NLRP12,apoptosis-related spot-like protein,Caspase-3 mRNA and protein in peripheral blood mononuclear cells of diabetes mellitus group,coronary heart disease group and diabetes mellitus complicated with coronary heart disease group were significantly higher than those of the control group(P<0.05).The apoptosis-related spot-like protein and Caspase-3 mRNA in peripheral blood mononuclear cells of diabetes mellitus complicated with coronary heart disease group were significantly higher than those of the control group(P<0.05).The relative expression of NLRP12 was significantly higher than that of diabetes mellitus group and coronary heart disease group(P<0.05).There was no significant difference in the relative expression of NLRP12 gene and protein between diabetes mellitus group,coronary heart disease group and diabetes mellitus complicated with coronary heart disease group(P>0.05).The plasma levels of IL-18 in diabetes mellitus group,coronary heart disease group and diabetes mellitus complicated with coronary heart disease group were significantly higher than those in the control group(P<0.01);the plasma levels of IL-18 in diabetes complicated with coronary heart disease group were significantly higher than those in diabetes mellitus group and coronary heart disease group(P<0.05).The correlation analysis showed that plasma IL-18 level was positively correlated with NLRP12,apoptosis-related spot-like protein,Caspase-3 mRNA and relative protein expression.Conclusion The abnormal activation of NLRP12 promotes the maturation and release of downstream inflammatory factor IL-18 by activating the expression of apoptosis related spot like protein and Caspase-3,and participates in the occurrence and development of diabetes mellitus complicated with coronary heart disease.
作者
张婧
隋国良
Zhang Jing;Sui Guoliang(Department of Endocrinology,Yantai Yantaishan Hospital,Yantai 264001,China)
出处
《中华内分泌外科杂志》
CAS
2020年第5期411-415,共5页
Chinese Journal of Endocrine Surgery
基金
山东省重点研发计划(2017GSF18173)。
作者简介
通信作者:张婧,Email:1182841927@qq.com,电话:13562592858。
