摘要
目的探讨miR-149通过BMP/Smad信号通路对舌鳞状细胞癌细胞增殖侵袭的影响。方法采用荧光定量PCR实验测定miR-149在舌鳞状细胞癌细胞(Tca8113和CAL-27)中的mRNA表达,采用MTT法检测miR-149过表达(miR-149-mim组)对Tca8113和CAL-27细胞增殖的影响;采用Transwell细胞侵袭实验检测miR-149过表达对Tca8113和CAL-27细胞侵袭的影响;采用Western blot检测BMP/Smad信号通路相关蛋白表达量。结果CAL-27和Tac8113细胞中的miR-149 mRNA表达水平相较ATCC细胞显著下调(t=3.562、3.642,P<0.05);miR-149-mim组CAL-27和Tac8113细胞增殖速度相较miR-149-con组显著下调(t=3.332、3.354,P<0.05);miR-149-mim组的CAL-27和Tac8113细胞侵袭个数相较miR-149-con组显著下调(t=3.884、3.892,P<0.05);miR-149-mim组的CAL-27和TAC8113细胞BMP-2、Smad1/5/8和p-Smad1/5/8表达水平相较miR-149-con组显著下调(t=3.243、3.196、3.368,P<0.05)。结论过表达miR-149可以通过激活BMP/Smad信号通路实现对舌鳞状细胞癌细胞增殖侵袭的抑制作用。
Objective To investigate the effect of miR-149 on the proliferation and invasion of tongue squamous cell carcinoma cells through BMP/Smad signal pathway.Methods Fluorescent quantitative PCR assay was used to detect the mRNA expression of miR-149 in tongue squamous cell carcinoma cells(Tca8113 and CAL-27),MTT was used to detect effect of miR-149 overexpression(miR-149-mim group)on the proliferation of Tca8113 and CAL-27 cells,Western Blot was used to detect the expression of proteins related to BMP/Smad signal pathway.Results The expression of miR-149 mRNA in CAL-27 and Tac8113 cells was significantly lower than that in ATCC cells(t=3.562 vs.t=3.642,P<0.05),and the proliferation rate of CAL-27 and Tac8113 cells in miR-149-mim group was significantly lower than that in miR-149-con group(t=3.332 vs.t=3.354,P<0.05).The expression levels of BMP-2,Smad1/5/8 and p-Smad1/5/8 in CAL-27 and TAC8113 cells in miR-149-mim group were significantly lower than those in miR-149-con group(t=3.243,3.368 and 3.196).Conclusion Overexpression of miR-149 can inhibit the proliferation and invasion of tongue squamous cell carcinoma cells by activating BMP/Smad signal pathway.
作者
李京洋
保森竹
姚毅章
黄文泉
LI Jingyang;BAO Senzhu;YAO Yizhang;HUANG Wenquan(The Fifth People′s Hospital of Qinghai Province,Qinghai Cancer Hospital,Xining 810000,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2020年第19期2630-2633,共4页
The Journal of Practical Medicine
基金
江西省卫生计生委科技计划课题(编号:20195349)。
