摘要
为探究鸭肠炎病毒(Duck enteritis virus,DEV)感染对鸭十二指肠黏膜组织不同时间段相关免疫基因的变化影响,采用DEV接种30日龄的健康樱桃谷鸭,在感染后72 h、96 h和120 h采集十二指肠,使用BGISEQ-500平台对3个试验组和对照组十二指肠组织测序,并筛选差异表达基因,用GO和KEGG数据库进行分析。结果显示,T72、T96和T120分别筛选出798、1389和1329差异表达基因;GO功能注释显示,差异基因主要参与到细胞因子活性、受体结合、细胞外基质、免疫系统过程、防御反应、钙离子结合、受体调节活性、受体配体活性、钠∶氨基酸转运体活性等;KEGG通路富集结果显示,差异表达基因主要涉及在细胞因子-细胞因子受体相互作用、NOD样受体信号通路、RIG-I样受体信号通路,抗原加工和呈递、Toll样受体信号通路、ECM-受体相互作用、PI3K-Akt信号通路、细胞溶质DNA传感途径、RIG-I样受体信号通路蛋白质消化吸收、细胞黏附分子(CAMs)、细胞色素P450对异生素的代谢、类固醇激素生物合成、PPAR信号通路Toll样受体信号通路、补体和凝血级联、IL-17信号通路、钠∶氨基酸转运体活性、神经递质转运蛋白活性、有机酸∶钠转运体活性、阳离子∶氨基酸转运体活性、膜的组成成分和膜的内在成分。用real-time PCR对差异表达基因的相对表达倍数进行验证,与RNA-seq测序结果基本一致。研究结果为探究DEV感染樱桃谷鸭十二指肠黏膜组织致病机制及其他生物学通路奠定了基础。
To investigate the effects of duck enteritis virus(DEV)infection on the changes of immune genes in duck duodenal mucosa at different time points of 72 h,96 h and 120 h,this experiment used DEV to inoculate 35-day-old healthy Cherry Valley ducks.The duodenum was collected at 72 h,96 h and 120 h after infection.The duodenum tissues of the three test groups and the control group were sequenced using the BGISEQ-500 platform.And screening for differentially expressed genes was analyzed using the GO and KEGG databases.The results showed that T72,T96 and T120 screened 798,1389 and 1329 differentially expressed genes respectively;GO function annotation showed that differential genes were mainly involved in cytokine activity,receptor binding,extracellular matrix,immune system processes,defense responses,calcium ion binding,receptor-modulating activity,receptor ligand activity,sodium:amino acid transporter activity,etc.;KEGG pathway enrichment results showed that cytokine-cytokine receptor interaction,NOD-like receptor signaling pathway,RIG-I-like receptor signaling pathway,antigen processing and presentation,Toll-like receptor signaling pathway,ECM-receptor interaction,PI3K-Akt signaling pathway,cytosolic DNA sensing pathway,RIG-I-like receptor signaling pathway protein digestion and absorption,cell adhesion molecules(CAMs),cytochrome P450 metabolism of xenobiotics,steroid hormone biosynthesis,PPAR signaling pathway Toll-like receptor signaling pathway,complement and coagulation cascade,IL-17 signaling pathway,sodium:amino acid transporter activity,neurotransmitter transporter activity,organic acid:sodium transporter activity,cation:amino acid transporter activity,membrane constituents and membrane intrinsic.The relative expression folds of differentially expressed genes were verified by q RT-PCR,and the results were basically consistent with the results of RNA-seq.The results laid the foundation for exploring the pathogenic mechanism and other biological pathways of DEV infection in the duodenal mucosa of Cherry Valley duck.
作者
张旭
郑敏
吴征卓
姚碧琼
施大军
周碧君
文明
程振涛
王开功
赵大杰
陈国权
田琴
阎朝华
ZHANG Xu;ZHENG Min;WU Zheng-zhuo;YAN Bi-qiong;SHI Da-jun;ZHOU Bi-jun;WEN Ming;CHEN Zhen-tao;WANG Kai-gong;ZHAO Da-jie;CHENG Guo-quan;TIAN Qing;YAN Chao-hua(College of Animal Science,Guiyang,Guizhou,550025,China;Guizhou Provincial Animal Disease Research Laboratory,Guiyang,Guizhou,550025,China;Key Laboratory of Animal Diseases and Veterinary Public Health of Guizhou Province,Guiyang,Guizhou,550025,China;Duck Industrialization Construction Management Office of Sansui County,Sansui,Guizhou,556500,China;Qianlishan Ecological food Company limited of Guizhou Province,Sansui,Guizhou,556500,China;Chongqing Three Gorges Vocational College,Chongqing,404155,China)
出处
《动物医学进展》
北大核心
2020年第10期73-79,共7页
Progress In Veterinary Medicine
基金
国家自然科学基金项目(31260607,31560703)
贵州省百层次创新型人才项目(黔科合人才[2016]4009号)
三穗鸭工程技术研究中心建设项目(黔科合平台人才[2019]5203号)
贵州省科技平台及人才团队计划项目(黔科合平台人才[2018]5253号)
贵州省研究生教育创新计划项目(GZZ201700)。
关键词
鸭肠炎病毒
十二指肠
转录组测序
Duck enteritis virus
duodenum
transcriptome sequencing
作者简介
张旭(1992-),男,贵州省正安人,硕士研究生,从事微生物与免疫学研究;通讯作者:周碧君;通讯作者:文明。
