摘要
为了探明侵蚀地施加菌糠后土壤理化性质及细菌群落结构的变化情况,采用细菌16S rRNA基因V3-V4区高通量测序技术等方法分析施加菌糠的大豆、太子参和玉米种植地与对照地土壤中细菌群落组成和多样性的差异,并分析土壤理化性质与细菌群落的关系。结果表明:施加菌糠能够一定程度地提高土壤的理化性质,并对土壤质量有所改善。施用菌糠的土壤样品中共检测到676属细菌,其中德沃斯氏菌属(Devosia,0.02%~3.81%)和Sideroxydans(0.01%~6.02%)为优势属;相较于对照地土壤,施加菌糠后土壤中18个属的群落结构发生显著变化。冗余分析表明,土壤中17个细菌属的丰度与毛管孔隙度、非毛管孔隙度、pH、有机质、全磷、全钾、碱解氮以及有效磷的含量呈显著相关关系。菌糠的施用改善了土壤的理化性质,增加了土壤中促进物质代谢和养分吸收的菌群丰度,为菌糠用作有机肥料的进一步开发研究提供了理论基础。
Based on the high-throughput sequencing technology of v3-v4 region of bacterial 16S rRNA gene,it was analyzed the differences of bacterial community composition and diversity in the soil of soybean,Pseudostellaria and maize between the planting sites and control sites,and then was analyzed the relationship between the physical&chemical properties of soil and bacterial community.The study was made to explore the changes of soil bacterial community composition and diversity after the application of spent mushroom substrate,and to reveal the influence of soil physical&chemical properties on the bacterial community composition.The results show that the physical and chemical properties of the soil were improved by the application of spent mushroom substrate.A total of 676 genera were detected in the soil samples,in the same time,the dominant genus were Devosia(0.02%-3.81%)and SIdeoxydans(0.01%-6.02%).Compared with the control soil,the community composition of 18 genera of bacteria in the soil changed significantly after the application of spent mushroom substrate.In addition,RDA analysis shows that the abundance of bacteria is significantly correlated with capillary porosity,non capillary porosity,pH,organic matter,total phosphorus,total potassium,alkali hydrolyzed nitrogen and available phosphorus.In conclusion,the application of mushroom substrate can improve the physical&chemical properties of soil,and increase the abundance of bacteria in the soil to promote material metabolism and nutrient absorption,which provides the theoretical basis for the further development and utilization of spent mushroom substrate as an organic fertilizer.
作者
林敬兰
毕小惠
唐丽芳
许欣
夏卫平
柳婷
孙淑静
Lin Jinglan;Bi Xiaohui;Tang Lifang;Xu Xin;Xia Weiping;Liu Ting;Sun shujing(Fujian Soil and Water Conservation Experimental Station,Fuzhou, Fujian Province, 350003;College of Life Science,Fujian Agriculture and Forestry University,Fuzhou, Fujian Province, 350002)
出处
《亚热带水土保持》
2020年第3期1-7,共7页
Subtropical Soil and Water Conservation
基金
福建省水利科技项目“食用菌菌糠改良劣质红壤性能研究”(MSK201822)。
关键词
菌糠
高通量测序
土壤理化性质
细菌多样性
细菌群落结构
mushroom substrate
high-throughput sequencing
soil physical&chemical property
bacterial diversity
bacterial community composition
作者简介
林敬兰(1970-),女,福建省浦城县人,高级农艺师,博士,主要从事水土保持科学和技术推广研究。
