摘要
近年来,农产品安全领域中的药物残留问题引起了人们的广泛关注,为了保障国家食品安全和国民健康、促进经济贸易发展,对农产品中的农药、抗生素等有害残留物进行检测是非常必要的。表面增强拉曼散射技术(SERS)作为一种新兴的检测手段,具有操作简单、耗时短、灵敏度高等优点。对SERS技术概况、增强理论和增强基底进行了简要介绍,以药物残留检测领域常用的金属溶胶类基底、固体活性基底和柔性活性基底为切入点,重点介绍了表面增强拉曼光谱技术在农产品(肉类、水产品、果蔬和其他部分农产品等)药物残留检测领域中的研究现状。传统溶胶基底具有成本低、易合成和SERS性能优异等特点,为了提高增强效果,众多研究者从尺寸和形态入手进行基底优化,开发出花状纳米结构、星状纳米结构、棒状纳米结构和链状纳米结构等形态的溶胶颗粒;基于复合材料互补协同效应发展了一系列的核壳纳米结构,有助于提升胶体基底的拉曼性能和稳定性。固体基底结构稳定,具有较好的一致性、重复性。柔性基底主要有基于柔性器件的、柔性聚合物的、以及柔性碳材料的三类SERS基底,其具有机械性能优良、不易损坏、成本低等优点,有利于实现微创或无损检测。通过对比分析,发现基底类型、待检农产品的基质、农药类型、检测环境均会对检测结果产生影响,当前研究的灵敏度较高,表明了SERS技术结合纳米基底在检测复杂基质中农药残留的应用潜力。同时指出应用SERS技术进行农产品药物残留检测的挑战:(1)农产品体内的药物残留量低、分布不均匀,导致拉曼信号相对较弱且易受荧光干扰和背景噪声干扰;(2)农产品基质复杂,对SERS光谱数据的影响不容小觑;(3)当前SERS检测方法尚未标准化,不同检测方案的结果差异较大。随着表面增强拉曼散射的支撑理论和检测技术的不断进步,SERS将在食品安全领域具有更广阔的应用前景。
Food safety issues,especially those related to pesticides and antibiotics residues,have aroused widespread concern in recent years.Therefore,the detection of pesticides,antibiotics and other harmful residues in agricultural products is an indispensable step for guaranteeing the national food safety,consumer's health and promoting economic and trade development.As an emerging detection method,Surface-enhanced Raman scattering(SERS)has the advantages of simple operation,short operation time and high sensitivity.A brief introduction on SERS,the SERS enhancement theory and SERS active substrate is given first of all.The paper focuses on the current research status of SERS in the field of pesticides residue detection in agricultural products(such as animal meat,aquatic products,fruits and vegetables and other agricultural products,etc.).Traditional colloids substrate has the characteristics of low cost,easy synthesis and excellent Performance of SERS.In order to improve the enhancement effect,many researchers optimize the size and morphology of the substrate to form flower-shaped nanostructure,star nanostructure,rod nanostructure and chain nanostructure and other forms of colloids particles.In order to improve the performance and stability of colloidal substrates,a series of core-shell nanostructures have been developed based on the synergy of composite materials.The solid substrate structure is stable and has good consistency and repeatability.There are three kinds of flexible substrates,SERS substrate based on flexible devices,SERS substrate based on flexible polymer and SERS substrate based on flexible carbon material,which has the advantages of excellent mechanical properties,not easy to damage and low cost,which is conducive to the realization of minimally invasive or non-destructive detection.The review summarized and compared these different studies of SERS methods,which could be used to detect pesticides,antibiotics and other harmful residues in agricultural products based on different substrates,different agricultural products,different pesticides.The sensitivity of the current study is relatively high,which shows the application potential of SERS technology combined with nano substrates in detecting pesticide residues in a complex matrix.At the same time,there are still great challenges for the application of SERS technology in the pesticides residue analysis.(1)Low pesticides residues and uneven distribution of pesticides in agricultural products lead to relatively weak Raman signals,which are susceptible to fluorescence and background noise.(2)The complex matrix of agricultural products has a great influence on the SERS spectral data.(3)The current SERS detection method has not been standardized,and the results of different detection schemes are quite different.Surface-enhanced Raman scattering,whether it is the detection technology or the support theory is progressing,are given for a broader application prospect in food safety.
作者
杨德红
张雷蕾
朱诚
YANG De-hong;ZHANG Lei-lei;ZHU Cheng(College of Life Sciences,China Jiliang University,Hangzhou 310000,China;Key Laboratory of Marine Food Quality and Hazard Control Technology in Zhejiang Province,Hangzhou 310018,China)
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2020年第10期3048-3055,共8页
Spectroscopy and Spectral Analysis
基金
浙江省自然科学基金青年基金项目(LQ18F050003)
国家重点研究与发展计划项目(2017YFF0211302)
浙江省专业学位研究生改革专项项目(Y201942783)资助。
关键词
表面增强拉曼光谱
活性基底
现场检测
农药
抗生素
Surface-enhanced Raman spectroscopy
Active substrates
On-spot detection
Pesticide
Antibiotic
作者简介
杨德红,1994年生,中国计量大学生命科学学院硕士研究生,e-mail:ydhzero@163.com;通讯联系人:朱诚,e-mail:pzhch@cjlu.edu.cn。
