摘要
为提高枯草芽孢杆菌SR096发酵液中芽孢含量,本研究通过单因素试验和响应面法试验对SR096发酵培养基中的碳源、氮源以及无机盐体系等组分进行了优化。最终确定发酵培养基组成为:葡萄糖7.5 g/L、玉米粉12.69 g/L、酵母粉10.66 g/L、黄豆粉20 g/L、柠檬酸钠4.46 g/L、碳酸钙1.5 g/L、硫酸镁2 g/L,优化后的发酵培养基在37℃、180 r/min条件下摇瓶培养48 h,枯草芽孢杆菌SR096菌株发酵液中芽孢含量可达7.978×10^9 cfu/mL,制备的益生菌剂具有耐热、耐酸、耐胆盐的优点并能在常温下长期贮存。
We tried to improve the spore content of Bacillus subtilis SR096 in its fermentation broth by optimize the medium formula.A response surface regression equation was established based on the results of one-factor-at-a-time,Plackett-Burman,steepest ascent and Box-Behnken experimental designs.The optimal medium formulation,consisted of 7.5 g/L glucose,12.69 g/L corn flour,10.66 g/L yeast extract powder,20 g/L soybean powde,4.46 g/L sodium citrate,1.5 g/L Ca CO3,2 g/L MgSO4. The yield of the spore reached 7.978×10^9 cfu/mL,which was 6.9-fold higher than that before the optimization by using the optimized medium at 37 ℃with shaking at a speed of 180 r/min for 48 h.The preparation of probiotics has the advantages of heat resistance,acid resistance,bile salt resistance and long-term storage at room temperature.
作者
郭建军
熊大维
曾静
魏国汶
杜建华
袁林
GUO Jianjun;XIONG Dawei;ZENG Jing;WEI Guowen;DU Jianhua;YUAN Lin(Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang,Jiangxi Province 330096,China;Nanchang Institute of Science and Technology,Nanchang,Jiangxi Province 330108,China)
出处
《中国饲料》
北大核心
2020年第19期16-22,共7页
China Feed
基金
江西省应用培育计划“饲用产酶枯草芽孢杆菌液体高密度发酵技术的研究与应用”(20181BBF68008)
江西省重点研发计划(20192BBF60029)。
关键词
枯草芽孢杆菌
发酵工艺
菌剂
保存稳定性
Bacillus subtilis
fermentation process
bacterium agent
stability during storage
作者简介
通讯作者:袁林。
