摘要
马铃薯淀粉废水中含有高浓度的淀粉、蛋白质等有机物。利用马铃薯淀粉废水培养植物促生菌,是实现其资源化利用的方法。采用单因素方法结合中心复合设计(CCD)的方法,对废水体积分数(浓度)、培养温度、初始pH这3个因素进行研究,优化马铃薯淀粉废水培养Paenibacillus polymyxa EBL06菌株的适宜生长条件。实验得到马铃薯淀粉废水培养P.polymyxa的最佳条件:废水COD为13.7 g·L^−1,初始pH为7.17,培养温度为31.4℃。该条件下,培养21 h后,微生物活菌数为6.2×10^9 cfu·mL^−1,与模型预测结果基本一致,可以达到《农用微生物菌剂国家标准》(GB 20287-2006)。为了验证该菌剂的应用效果,进行了蔬菜种植实验。结果表明:P.polymyxa菌剂能有效提高小白菜的产量与品质,作物鲜重、干重、株高,以及维生素C含量别提高了68.6%、13.7%、5.6%、41.3%;相比于只施用化肥的组,菌剂同尿素的混施能提高氮肥的利用效率,小白菜植株中维生素含量提高了25.3%、硝酸盐含量减少了15.3%。以上研究结果可为马铃薯淀粉废水的资源化利用,以及P.polymyxa菌剂的应用推广提供参考。
Potato starch wastewater contains high concentration of starch,protein and other organic matter.Using potato starch wastewater to cultivate plant growth-promoting bacteria is a method to realize its resource utilization.The growth conditions of Paenibacillus polymyxa EBL06 strain cultured in potato starch wastewater were optimized using a comibined single-factor and central composite design(CCD)approach,in which three key factors,i.e.,wastewater volume fraction(concentration),culture temperature and initial pH,were focused.In this context,the optimum conditions for the culture of P.polymyxa obtained from the experiment were as follows:COD concentration 13.7 g·L^−1,initial pH 7.17,culture temperature 31.4℃.Under this condition,the number of living bacteria was 6.2×10^9 cfu·mL^−1,which was consistent with the CCD predictions,and satisfying the national standard of agricultural microbial agents(GB 20287-2006).To verify the application effect of the microbial agent,the experiment of vegetable planting was then carried out.The results showed that the P.polymyxa biofertilizer could significantly increase the yield and quality of pakchoi;its fresh weight,dry weight,plant height and vitamin C content increased by 68.6%,13.7%,5.6%and 41.3%,respectively;compared with the fertilization of chemical fertilizer only,the mixed fertilization of P.polymyxa biofertilizer and urea fertilizer could improve the nitrogen utilization efficiency,and resulted in an increment in the content of vitamin in pakchoi plant by nearly 25.3%,and meanwhile,contributed to an decrease in the content of nitrate decreased by 15.3%.Overall,this study provides a meaningful reference for the resource utilization of potato starch wastewater,as well as the application and popularization of P.polymyxa.
作者
刘浩
李瑞
包丽君
王分分
张旭坡
曲东
白志辉
LIU Hao;LI Rui;BAO Lijun;WANG Fenfen;ZHANG Xupo;QU Dong;BAI Zhihui(College of Resource and Environment,Northwest A&F University,Yangling 712100,China;State Key Laboratory of Environmental Aquatic Chemistry,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Key Laboratory of Environmental Biotechnology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;College of Environment&Resources,University of Chinese Academy of Sciences,Beijing 100049,China;College of Environmental Science and Engineering,Hebei University of Science and Technology,Shijiazhuang 050018,China)
出处
《环境工程学报》
CAS
CSCD
北大核心
2020年第9期2406-2415,共10页
Chinese Journal of Environmental Engineering
基金
国家水体污染控制与治理科技重大专项(2018ZX07110)
国家重点研发计划项目(2016YFC0501400)。
作者简介
第一作者:刘浩(1988—),男,博士研究生。研究方向:微生物肥料。E-mail:lh880330@qq.com;通信作者:白志辉(1971—),男,博士,研究员。研究方向:环境生物技术。E-mail:zhbai@rcees.ac.cn。
