摘要
建立测定饮料、配制酒、干制豆制品中胭脂红、日落黄、诱惑红、亮蓝、酸性红、碱性橙2、碱性橙21、碱性橙22、酸性橙2、碱性嫩黄O的高效液相色谱法。以V(甲醇)∶V(水)=8∶2为萃取溶剂对样品中色素直接萃取,高效液相色谱仪配二极管阵列检测器进行测定,色谱柱Agela Venusil XBP-C 18(250 mm×4.6 mm,5μm)进行分离;449、485、254、628 nm为最佳吸收波长;柱温25℃;流速1 mL/min;流动相20 mmol/L醋酸铵溶液-甲醇进行洗脱。经方法学验证,色谱峰分离度均>1.5,在线性范围0.5~125 mg/kg内相关系数均>0.999,液体样品加标回收率均>85%,固体样品加标回收率均>75%。以饮料为基质进行加标,计算得出10种色素的日内精密度为0.76%~4.10%(n=6),日间精密度为1.09%~5.29%(n=6)。结果表明,该方法准确性、灵敏度、精密度良好,可满足食品检测行业对其检测的要求。
The detection method of carmine,sunset yellow,allure red,brilliant blue,acid red,basic orange 2,basic orange 21,basic orange 22,acid orange 2,auramine O in beverages,prepared wines,and dried soy products O using high performance liquid chromatography were established.Methanol:water(8∶2)were used as the extraction solvent to directly extract the pigment from the sample.The high-performance liquid chromatograph equipped with a diode array detector for the determination and the column was Agela Venusil XBP-C 18 XBP-C 18(250 mm×4.6 mm,5μm).The following condition was used in this study including 449 nm,485 nm,254 nm,and 628 nm absorption wavelengths;column temperature at 25°C with 1 mL/min flow rate.Mobile phase:20 mmol/L ammonium acetate solution-methanol for elution.According to the methodological verification,all the chromatographic peak resolutions were>1.5.The correlation coefficients within the linear range of 0.5 mg/kg-125 mg/kg were all>0.999;the recovery rate of the liquid samples was>85%and the recovery rate of the solid samples was>75%.Based on the calculation of the standard addition of beverages,the intra-day precision(RSD%)of 10 pigments was 0.76%-4.10%(n=6),and the intra-day precision(RSD%)was 1.09%-5.29%(n=6).The results showed that the method had good accuracy,sensitivity and precision,and could meet the testing requirements of the food testing industry.
作者
郭永辉
赵连兴
柯泽华
张纪英
刘贵巧
GUO Yonghui;ZHAO Lianxing;KE Zehua;ZHANG Jiying;LIU Guiqiao(College of Life Science and Food Engineering,Hebei University of Engineering,Handan 056000,China;Food and Drug Inspection Center of Handan City,Handan 056000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第17期259-263,270,共6页
Food and Fermentation Industries
关键词
液相色谱
人工合成着色剂
工业染料
直接萃取
饮料
干制豆制品
liquid chromatography
synthetic colorants
industrial dyes
direct extraction
beverages
dried soy products
作者简介
第一作者:郭永辉,硕士研究生;通讯作者:张纪英,高级实验师,E-mail:Zhangjiying8576172@163.com。
