摘要
目的探讨桑葚水提物对H2O2诱导后大鼠骨髓间充质干细胞(BMSCs)成脂分化的影响,并初步探讨其机制。方法流式细胞术鉴定分离培养的大鼠BMSCs;真空冷冻干燥法制备桑葚水提物,CCK-8法检测桑葚水提物(15、30、60 mg/L)对BMSCs生长的影响;油红O染色显示桑葚水提物(15、30、60 mg/L)对100μmol/L H2O2诱导培养的BMSCs成脂分化的影响;免疫荧光、Western blot检测60 mg/L桑葚水提物作用于H2O2诱导后BMSCs中过氧化物酶体增殖物激活受体γ(PPARγ)和CCAAT/增强子结合蛋白α(C/EBPα)表达的影响。结果不同浓度桑葚水提物对BMSCs生长无影响,各组间差异无统计学意义(P>0.05)。桑葚水提物可抑制H2O2诱导后BMSCs中脂滴生成,呈现一定的浓度依赖趋势,差异有统计学意义(P<0.01)。免疫荧光、Western blot结果表明60 mg/L桑葚水提物可抑制PPARγ和C/EBPα的表达,与模型组相比,差异有统计学意义(P<0.05)。结论桑葚水提物可通过抑制PPARγ和C/EBPα减少H2O2诱导的BMSCs成脂分化。
Objective To investigate the effect of the aqueous extracts of mulberry on adipogenic differentiation of bone marrow mesenchymal stem cells(BMSCs) stimulated by H2O2.Methods BMSCs were isolated from the bone marrow of rats and identified by flow cytometry. The aqueous extracts of mulberry were prepared by vacuum freeze-drying. CCK-8 method was performed to detect the toxicity of mulberry aqueous extract(15, 30, 60 mg/L) to BMSCs. Oil red O staining was used to identify the lipid droplets. Immunofluorescence and Western blot were used to detect the expression of PPARγ and C/EBPα in BMSCs treated by the aqueous extracts of mulberry(15, 30, 60 mg/L).Results CCK-8 assay showed that the aqueous extracts of mulberry did not affect the growth of BMSCs(P>0.05). Oil red O staining showed that the aqueous extracts of mulberry inhibited the formation of lipid droplets in BMSCs induced by H2O2, showing a dose-effect relationship(P<0.01). The aqueous extracts of mulberry(60 mg/L) significantly inhibited the expression of PPARγ and C/EBPα in BMSCs stimulated by H2O2(P<0.05).Conclusion The aqueous extracts of mulberry can inhibit the adipogenic differentiation of BMSCs induced by H2O2 trough PPARγ and C/EBPα signaling pathway.
作者
靳俊峰
刘银花
欧小波
阮媛
王海青
陈燕玲
吴秀香
易华
Jin Junfeng;Liu Yinhua;Ou Xiaobo(Dept of Pathology,Zhuhai 519041;Biological Medicine R&D Center 9 Zhuhai Campus Zunyi Medical University,Zhuhai 519041)
出处
《安徽医科大学学报》
CAS
北大核心
2020年第7期1098-1102,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81403202)
广东省医学科研基金项目(编号:A2012620)。
作者简介
靳俊峰,男,副教授,硕士;易华,女,副教授,硕士生导师,责任作者:E-mail:yingying020@gzucm.edu.cn。
