摘要
目的:探究与骨质疏松相关的microRNA的生物学作用,并初步验证microRNA在氧化应激状态下在成骨细胞中的作用。方法:骨质疏松患者的microRNA芯片数据选自GEO数据库,使用GEO2R分析骨质疏松与非骨质疏松之间差异表达的microRNA,miRDB和miRTarBase数据库预测差异microRNA的靶基因并进行GO富集分析和KEGG通路分析,同时建立小鼠骨质疏松动物模型及处于氧化应激状态的成骨细胞,使用qPCR检测相关microRNA的表达。结果:从GSE74209数据集中筛选出│log(foldchange)│>2的microRNA26个,靶基因进行KEGG通路分析发现黏着斑、MAPK通路、激动蛋白骨架调节、趋化因子信号通路等与骨质疏松的发生密切相关,动物模型中检测到miR-491-3p、miR-22-3p、miR-223-3p、miR-32-3p、miR-30c-1-3p、miR-26b-5p表达均降低,氧化应激状态下成骨细胞中检测到miR-30c-1-3p、miR-32-3p表达降低,miR-32-3p与芯片分析结果一致。结论:miR-32-3p可能通过调节成骨细胞的氧化应激反应,在骨质疏松中发挥重要作用。
Objective:To explore the biological function of microRNA related to osteoporosis,and to verify the role of microRNA in osteoblasts under oxidative stress initially.Methods:MicroRNA microarray data of osteoporosis patients were selected from GEO database,and GEO2R was used to analyze microRNA differentially expressed between osteoporosis and non-osteoporosis.Target genes were predicted by miRDB and miRTarBase,that were imported into DAVID database for GO enrichment analysis and KEGG pathway analysis.Mice osteoporosis animal model and osteoblast under oxidative stress were established,and qPCR was used to detect the expression of relevant microRNA.Results:26 microRNAs of│log(fold change)│>2 were selected from the GSE74209 dataset.KEGG pathway analysis of target genes showed that focal adhesion,MAPK signaling pathway,regulation of actin cytoskeleton,chemokine signaling pathway,etc.,were closely related to the occurrence of osteoporosis.MiR-491-3p,miR-22-3p,miR-223-3p,miR-32-3p,miR-30c-1-3p,and miR-26b-5p were all decreased in animal models.MiR-30c-1-3p and miR-32-3p were decreased in the oxidative osteoblasts.MiR-32-3p expression level was consistent with the results of microarray analysis.Conclusions:miR-32-3p may play an important role in osteoporosis by regulating the oxidative stress in osteoblasts.
作者
于淼
李生娇
艾泽馨
吴洋欧
李家
YU Miao;LI Sheng-jiao;AI Ze-xin;WU Yang-ou;LI Jia(Shanghai Engineering Research Center of Tooth Restoration and Regeneration,Department of Oral and Maxillofacial Surgery,School of Hospital of Stomatology,Tongji University,Shanghai 200072,China)
出处
《中华老年口腔医学杂志》
2020年第4期193-199,共7页
Chinese Journal of Geriatric Dentistry
基金
上海市科学技术委员会项目(项目编号:19140904800,16ZR1439700)。
作者简介
于淼,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学附属口腔医院口腔颌面外科教研室硕士,上海200072;李生娇,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学附属口腔医院口腔颌面外科教研室副教授,上海200072;艾泽馨,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学附属口腔医院口腔颌面外科教研室硕士,上海200072;吴洋欧,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学附属口腔医院口腔颌面外科教研室硕士,上海200072;通讯作者:李家,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学附属口腔医院口腔修复教研室副主任医师,上海200072。
