摘要
目的确认一份多品系混杂转基因大豆样品中是否含有复合性状转基因大豆。方法采用实时荧光定性PCR方法对实验室留存的一份转基因初筛阳性大豆样品进行品系筛查检测,后分别采用单粒多靶点筛查检测和清洗后单粒多靶点筛查检测方法进行复合性状品系的确认。结果经鉴定,该大豆样品中混杂了5种转基因大豆品系,检出复合性状转基因大豆为MON87708×MON89788品系,该品系为中国未经批准进口的转基因大豆品系。结论鉴于目前缺乏完整的检测技术体系和技术标准,这种多品系混杂样品中掺杂未批准复合性状品系的行为非常具有隐蔽性和欺骗性,国内的农业安全监管部门和海关检疫部门都应该对此高度重视。
Objective To confirm whether a mixed transgenic soybean sample contains stacked-traits event. Methods A multi-events mixed transgenic soybean sample was detected by real-time fluorescence qualitative PCR, and then single seed for multiple targets screening method and single seed for multiple targets screening after cleaning method were used to confirm the GM stack soybean. Results Five transgenic soybean events were identified, which were identified as MON87708×MON89788, an unapproved transgenic soybean event in China. Conclusion In view of the lack of complete detection technology and technical standards, the behavior of adulterating unapproved genetically modified stacked events in mixed transgenic soybean samples is very hidden and deceptive, so the domestic agricultural safety supervision department and the customs should attach great importance to it.
作者
徐君怡
秦朝秋
白景莲
白璐
刘莹
王琦
王玫
XU Jun-Yi;QIN Zhao-Qiu;BAI Jing-Lian;BAI Lu;LIU Ying;WANG Qi;WANG Mei(Technology Center of Dalian Customs District,Dalian 116001,China)
出处
《食品安全质量检测学报》
CAS
2020年第15期5287-5293,共7页
Journal of Food Safety and Quality
基金
国家转基因重大专项(2018ZX08012-001)。
关键词
转基因大豆
品系筛查
复合性状
transgenic soybean
event screening
stacked-traits
作者简介
徐君怡,研究员,主要研究方向为食品安全检测。E-mail:33348249@qq.com;通讯作者:王玫,研究员,主要研究方向为海关科技管理。E-mail:lnciqwangmei@163.com。
