摘要
目的建立气相色谱-负化学源质谱法(gaschromatography-negativechemicalionization,GC-MS/NCI)测定果蔬中37种农药残留量的方法。方法蔬菜水果样品由乙腈提取、QuEChERS净化,采用GC-MS/NCI测定,外标法定量。结果在20~500μg/L范围内37种农药的线性良好(相关系数r2>0.995),所有农药的方法定量限在0.03~0.67μg/kg之间;芹菜和梨在0.01 mg/kg加标水平下, 37种农药的平均回收率范围为86.2%~110%且相对标准偏差(n=6)均小于10%。结论该方法简便、快捷、回收率及精密度好,可以成为检测果蔬中农药残留的常规检测技术,其建立的质谱条件也可以成为农药确证的另一手段。
Objective To establish a gas chromatography-negative chemical ionization mass spectrometry(GC-MS/NCI) method for the determination of 37 pesticide residues in fruits and vegetables. Methods The fruit and vegetable samples were extracted with acetonitrile, cleaned up with QuEChERS, detected by GC-MS/NCI, and quantified by external standard method. Results The linearity of 37 pesticides was good in the range of 20–500 μg/L(correlation coefficient r2>0.995), and the quantitative limit of all pesticides was 0.03–0.67 μg/kg. The average recoveries of 37 pesticides in celery and pear were 86.2%–110% and relative standard deviations(n=6) were less than 10% at 0.01 mg/kg standard addition level. Conclusion The method is simple, rapid, and has good recovery and precision. It can be used as a routine detection technology for pesticide residues in fruits and vegetables, and the mass spectrometry conditions established by the method can also be used as another means for pesticide confirmation.
作者
李燕妹
连增维
LI Yan-Mei;LIAN Zeng-Wei(Fujian Inspection and Research Institute for Product Quality,Fuzhou 350002,China;Jike Testing Technology Co.,Ltd.(Fujian),Zhangzhou 353000,China)
出处
《食品安全质量检测学报》
CAS
2020年第15期5074-5079,共6页
Journal of Food Safety and Quality
关键词
果蔬
QUECHERS
农药残留
气相色谱-负化学源质谱法
vegetables and fruits
QuEChERS
pesticide residues
gas chromatography-negative chemical ionization
作者简介
李燕妹,硕士,助理工程师,主要研究方向为食品安全检测。E-mail:1532792074@qq.com;通讯作者:连增维,主要研究方向为食品质量控制与分析测试。E-mail:lianzengwei@qq.com。
