摘要
目的:探讨经颅直流电刺激(tDCS)治疗高血压的中枢神经系统机制。方法:雄性SD大鼠(180~200 g)随机分为对照组和应激性高血压模型组,通过给予模型组大鼠噪声和足底电击刺激制作应激性高血压大鼠模型,采用尾部套管法检测血压,运用碳纤维电极检测技术测定大鼠电刺激蓝斑核,下丘脑内去甲肾上腺素(NE)的释放量,进一步观察麻醉状态下tDCS(正极置于左侧前额叶皮质,负极置于右侧前肢)对NE释放量的影响。结果:高血压模型组大鼠造模后血压升高(P<0.05)。tDCS(tDCS 2 mA/20 min,每天2次,连续5 d)使模型组1大鼠血压下降(P<0.05),而接受假tDCS(tDCS 0 mA/20 min,每天2次,连续5 d)的模型组2大鼠血压没有下降(P>0.05)。长时程tDCS(2 mA/20 min,每天2次,连续5 d),或者在体tDCS(2 mA/20 min)都可使高血压模型组大鼠下丘脑NE的释放信号幅度减小(P<0.05)。结论:tDCS可缓解应激性高血压模型大鼠的血压升高,这种作用与tDCS减少蓝斑到下丘脑投射系统NE的分泌有关。
Objective:To observe the effects of transcranial direct current stimulation(tDCS)on norepinephrine(NE)release in the hypothalamus of the stress-induced hypertension model rats for the central nervous system mechanism of tDCS in the treatment of hypertension.Methods:Sprague-Dawley(180-200 g)rats were randomly divided into hypertension model group and control group.Rat models of hypertension were developed by noise stimulation and electric foot-shock stress.Blood pressure in the rats was measured by tail cuff method.NE release at the locus coeruleus(LC)in hypothalamus following electric stimulation was recorded by carbon fiber electrode.tDCS(2mA/20min twice per day lasting for 5 days)was applied by positioning anode on left prefrontal cortex and cathode on right fore limb under anesthesia.Results:The blood pressure was significantly elevated in rats in the model group(P<0.05).Blood pressure was decreased after tDCS(2 mA/20 min twice per day lasting for 5 days)in rats from hypertension group 1(P<0.05),yet had no change in rats in the hypertension model group 2 by sham tDCS(0 mA/20min twice per day lasting for 5 days).Either long-term tDCS(in vivo 2 mA/20 min)or short-term(2 mA/20 min)resulted in reduced NE release in hypertension rat models(both P<0.05).Conclusions:The effects of tDCS decreasing hypertension in stress-induced hypertension model rats may be related to the decrease of the NE release in the hypothalamus that receives projection from the locus coeruleus.
作者
徐贤慧
刘沐熙
王峰
汪萌芽
黄宏平
XU Xianhui;LIU Muxi;WANG Feng;WANG Mengya;HUANG Hongping(Department of Physiology and Neurobiology,School of Humanity and Management,Wannan Medical College,Wuhu 241002,China)
出处
《皖南医学院学报》
CAS
2020年第4期307-310,共4页
Journal of Wannan Medical College
基金
国家自然科学基金项目(30770674)
安徽省优秀青年科技基金项目(08040106817)
皖南医学院博士启动基金(201206)。
关键词
经颅直流电刺激
应激性高血压大鼠
去甲肾上腺素
下丘脑
transcranial direct current stimulation
stress-induced hypertension rats
norepinephrine
hypothalamus
作者简介
徐贤慧(1993-),女,2017级硕士研究生(电话)17856923130,(电子信箱)2524015639@qq.com;通信作者:黄宏平,女,教授,硕士生导师,(电子信箱)huanghp@wnmc.edu.cn。
