摘要
目的:探讨雌激素对消化性溃疡模型小鼠组织损伤的影响及作用机制。方法:36只健康C57BL/6雌性小鼠适应性饲养7 d后随机分为假手术对照组、假手术+PU模型对照组、假手术+PU模型+雌激素干预组、去势对照组、去势+PU模型对照组、去势+PU模型+雌激素干预组,每组各6只。根据实验设置分别建立消化性溃疡模型以及进行卵巢去势手术,雌激素干预组注射500μg/kg雌激素,对照组注射等量生理盐水。干预7 d后,分别检测并计算胃溃疡指数和溃疡抑制率、测定小鼠胃液和十二指肠液pH以及胃蛋白酶活性、试剂盒检测各组小鼠血清中活性氧(ROS)和谷胱甘肽过氧化物酶(GSH-PX)活性以及丙二醛(MDA)的含量、小鼠ELISA试剂盒检测小鼠血清中IL-17和IL-25含量。结果:与假手术对照组相比,假手术+PU模型对照组的溃疡指数、出血点数、ROS活性、MDA含量以及IL-17和IL-25含量均显著增加,胃液pH、十二指肠液pH、GSH-Px活性均显著降低(P<0.05);与去势对照组相比,去势+PU模型对照组的变化趋势与上述一致,差异也均具有统计学意义(P<0.05);而分别与假手术+PU模型对照组和去势+PU模型对照组相比,两个雌激素干预组以上所有指标变化趋势与上述相反(P<0.05)。与去势假手术组相比,去势组溃疡指数、出血点数、ROS活性、MDA含量以及IL-17和IL-25含量均显著增加,胃液pH、十二指肠液pH、GSH-Px活性均显著降低(P<0.05)。结论:采用雌激素治疗可显著减少消化性溃疡小鼠溃疡指数和出血点数,进而使得小鼠胃液和十二指肠液pH升高,促进消化性溃疡小鼠症状的改善,其作用机制可能与改善氧化应激和炎性相关指标有关。
Objective:To investigate the effect of estrogen on tissue damage of peptic ulcer model mice and its related mechanism.Methods:Healthy C57BL/6 female mice were selected and divided into sham surgery control group,sham surgery+PU control group,sham surgery+PU+E2 group,castration control group,castration+PU control group,castration+PU In the E2 group,6 cases in each group.A peptic ulcer model and ovarian castration surgery were established according to the experimental settings.The estrogen intervention group was injected with 500μg/kg estrogen,and the control group was injected with the same amount of normal saline.After 7 days of intervention,gastric ulcer index,ulcer inhibition rate,pH and pepsin activity of gastric juice and duodenal fluid were measured,and the activities of reactive oxygen species(ROS),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in serum were detected by kit,and the contents of IL-17 and IL-25 in serum were detected by ELISA kit.Results:Compared with the sham operation group,the ulcer index,bleeding points,ROS activity,MDA content,and IL-17 and IL-25 contents in the sham operation+PU group increased significantly.The gastric juice pH,duodenal fluid pH and GSH-Px activities were significantly reduced(P<0.05).Compared with the castration control group,the change trend of the castration+PU group was consistent with the above,and the differences were also statistically significant(P<0.05),compared with the sham surgery+PU control group and the castration+PU control group,respectively,the changes in all indicators of the two E2 treatment groups were opposite to those described above(P<0.05).Compared with the castration sham operation group,the ulcer index,bleeding points,ROS activity,MDA content,and IL-17 and IL-25 content in the castration group were significantly increased,and the gastric juice pH,duodenal juice pH,and GSH-Px activity were significantly reduced(P<0.05).Conclusion:Treatment with estrogen can significantly reduce the ulcer index and bleeding points of peptic ulcer mice,which can increase the pH of gastric juice and duodenal juice in mice,and improve the symptoms of peptic ulcer mice.The mechanism may be related to improving stress and inflammation.
作者
郭艳红
姚青林
张雪丽
靳斌
GUO Yan-hong;YAO Qing-lin;ZHANG Xue-li;JIN Bin(Department of Gastroenterology,1.Xi'an First Hospital,Xi'an 710002;Tangdu Hospital,Xi'an 710038,Shaanxi,China)
出处
《川北医学院学报》
CAS
2020年第4期555-558,567,共5页
Journal of North Sichuan Medical College
基金
陕西省自然科学基金(2015JQ4044)。
作者简介
郭艳红(1974-),女,主治医师。E-mail:Guoyanhong2578@163.com;通讯作者:姚青林。E-mail:Guoyanhong2578@163.com。
