摘要
Gynogenesis is an important breeding method in aquaculture and has been widely applied to many fish species.If gynogenetic progenies are to inherit paternal partial genomic DNA,this will increase genetic variation and will provide a useful outcome for breeding.In this study,we investigated the genetic variation in homeobox(Hox)gene clusters(Hox A4 a,Hox A9 a,Hox A11 b,Hox B1 b,Hox C4 a,Hox C6 b,and Hox D10 a)among koi carp(Cyprinus carpio haematopterus,KOC;the stimulation sperm source),grass carp(Ctenopharyngodon idellus),and gynogenetic grass carp(GGC).We found paternal DNA(a special DNA fragment and Hox C6 b)derived from KOC and a recombinant gene belonging to Hox C6 b in GGC.We are the first to report the recombinant Hox C6 b in GGC.Our study provides further evidence for paternal DNA transmission to gynogenetic progenies,which is a finding with great significance for the genetic breeding of fish.
基金
supported by the National Natural Science Foundation of China(31802287,31730098,and 31430088)
the China Postdoctoral Science Foundation(2018M632969)
the earmarked fund for China Agriculture Research System(CARS-45)
the Key Research and Development Program of Hunan Province(2018NK2072)
the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province(20134486)。
作者简介
Zhuangwen Mao,Contributed equally to this work;Yeqing Fu,Contributed equally to this work;Shi Wang,Contributed equally to this work;Yude Wang,Contributed equally to this work;Corresponding author:Shaojun Liu(email:lsj@hunnu.edu.cn)。
