摘要
目的:初步分析大鼠口服双参颗粒散剂与水煎剂后的入血成分差异。方法:采用LC/MS-IT-TOF方法对双参颗粒醇提取物、水提取物含药血清、散剂含药血清进行分析,根据保留时间、精确相对分子质量,质谱数据等完成各组成分差异比较。结果:和水煎剂比较,初步鉴定出双参颗粒散剂特有的4个原型吸收入血成分,分别是Ginsenoside-Rh1,Ginsenoside-Rg2,Ginsenoside-Rb2和Ginsenoside-Rg3。结论:双参颗粒散剂和水煎剂在大鼠入血成分存在差异,这些入血成分可能是双参颗粒散剂异于水煎剂在血中起直接作用的物质。
Objective:To preliminary analyze the plasma effective components Difference of rats after intragastric administration of Shuangshen Granules(SSG).Methods:The liquid-mass spectrometer-ion trap-time-of-flight mass spectrometry(LC/MS-IT-TOF)method was used to analyze the medicated serum of Shuangshen Granules pulvis and water decoction,according to the retention time,accurate relative molecular mass,mass spectrometry data,etc.the components differences between groups were compared.Results:Compared with water decoction,there are 4 plasma effective components of Shuangshen Granules Pulvis:Ginsenoside-Rh1,Ginsenoside-Rg2,Ginsenoside-Rb2 and Ginsenoside-Rg3.Conclusion:Different plasma effective components were found between pulvis and water decoction of SSG after intragastric administration by rats,different from water decoction,these plasma effective components may be substances in which pulvis plays a direct role in blood.
作者
魏华民
章从恩
李杨帆
吴昊
俞静
林海
WEI Huamin;ZHANG Congen;LI Yangfan;WU Hao;YU Jing;LIN Hai(Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)
出处
《世界中医药》
CAS
2020年第16期2359-2363,共5页
World Chinese Medicine
基金
国家自然科学基金项目(81904102)
北京市自然科学基金项目(7184203)。
作者简介
魏华民(1984.09—),男,博士,主治医师,研究方向:中医肿瘤,E-mail:weihuamin6255@126.com;通讯作者:俞静(1975.08—),女,博士,主任医师,研究方向:恶性肿瘤的综合治疗,E-mail:yujing026@163.com;通讯作者:林海(1971.03—),男,硕士,副主任医师,研究方向:中医药治疗肿瘤,E-mail:lh_js@sina.com。
