摘要
【目的】从北方寒地种植的不同农作物根际土壤中分离高效解磷的细菌,为微生物制剂和磷肥的开发提供适于本地区的优良菌种。【方法】通过初筛和复筛从26株解磷菌中筛选获得一株高效解磷细菌,对其进行生理生化和分子生物学鉴定,同时采用钼蓝比色法测定解磷能力。采用平板对峙法测定拮抗植物病原菌能力。【结果】通过筛选后获得的菌株B51-7经鉴定为伯克霍尔德菌属。菌株在发酵液中可溶性磷含量最高达到832.74 mg/L,同时具有很强的广谱抑菌作用,抑菌率最高为89.71%,可以显著促进水稻生长。【结论】菌株B51-7是一株具有生物防治作用的高效解磷细菌,可应用于生物菌肥和生防制剂中。
[Objective]In order to find strains suitable for the development of biocontrol agents and biophosphate fertilizer,bacteria with high phosphorus-dissolving efficiency were isolated from the rhizosphere soils of different crops planted in the cold region of north China.[Methods]A highly effective phosphorous-dissolving bacterium was obtained from 26 bacterial strains with phosphorous-dissolving activity through preliminary and second screening.The bacterial strain was identified through physiological,biochemical and molecular biological methods,and its phosphorus-dissolving activity was tested by molybdenum blue colorimetry method.Meanwhile,the antagonism activity to several plant pathogens was determined by plate confrontation method.[Results]Strain B51-7 was obtained by the screening and identified as Burkholderia sp..The soluble phosphorus content of the strain B51-7 in the fermentation broth reached 832.7 mg/L.Meanwhile,this strain had a broad-spectrum antifungal activity with the highest antagonism rate of 89.71%.A pot experiment showed that the growth of rice was significantly promoted by inoculation of strain B51-7.[Conclusion]Strain B51-7 was a highly effective phosphate-dissolving bacterium with biological control effect,and could be applied for making biological fertilizers and biocontrol agents.
作者
朱德旋
杜春梅
董锡文
薛春梅
王瑞
Dexuan Zhu;Chunmei Du;Xiwen Dong;Chunmei Xue;Rui Wang(College of Life Sciences,Jiamusi University,Jiamusi 154007,Heilongjiang Province,China;Institute of Applied Microbiology,Jiamusi University,Jiamusi 154007,Heilongjiang Province,China)
出处
《微生物学报》
CAS
CSCD
北大核心
2020年第8期1672-1682,共11页
Acta Microbiologica Sinica
基金
佳木斯大学校长创新创业基金(XZYF2019-29)
北药与功能食品特色学科建设项目(2017)
黑龙江省自然科学基金(C2017064)。
关键词
寒地
解磷菌
植物病原菌
拮抗作用
cold region
phosphorus bacteria
plant pathogens
antagonism activity
作者简介
通信作者:杜春梅,Tel/Fax:+86-454-8603986,E-mail:dcm_037@163.com;通信作者:董锡文,Tel/Fax:+86-454-8617776,E-mail:dongxiwen@126.com。
