摘要
目的探讨神经激肽1受体(neurokinin 1 receptor,NK-1R)拮抗剂WIN 62,577是否对白细胞介素(interleukin,IL)-13诱导的支气管上皮细胞氧化应激损伤有保护作用。方法培养人支气管上皮细胞(16HBE),分为4组:对照组,IL-13组,IL-13+SP组[IL-13+感觉神经肽P物质(SP)],IL-13+WIN 62,577组。IL-13组给予IL-13(25 ng/ml)处理48 h,48 h后IL-13+SP组给予感觉神经肽SP(10 nmol/L)处理1 h,IL-13+WIN 62,577组给予WIN 62,577(10 nmol/L)处理1 h。检测各组细胞增殖活性,活性氧(reactive oxygen species,ROS)水平、丙二醛(malondialdehyde,MDA)含量及总超氧化物歧化酶(superoxide dismutase,SOD)活性。结果与对照组比较,IL-13组细胞增殖活力减低(P<0.001)、ROS水平增高(P=0.001)、MDA含量增高(P<0.001)、SOD活性降低(P<0.001),差异均有统计学意义;与IL-13组比较,给予感觉神经肽SP刺激后,细胞增殖活力减低(P=0.016)、ROS水平增高(P=0.031)、MDA含量增高(P<0.001)、SOD活性降低(P=0.011);而给予WIN 62,577干预可抑制IL-13诱导的人支气管上皮细胞增殖活力降低(P=0.018)、ROS水平增高(P=0.018)、MDA含量增高(P<0.001)、SOD活性降低(P=0.001)。结论感觉神经肽SP可加重IL-13诱导人支气管上皮细胞氧化应激损伤,NK-1R拮抗剂WIN 62,577可抑制IL-13诱导人支气管上皮细胞氧化应激损伤。
Objective To investigate whether WIN 62,577 which is an antagonist of neurokinin 1 receptor(NK-1R)has protective effects on interleukin(IL)-13-induced bronchial epithelial cell oxidative stress injury.Methods Human bronchial epithelial cells(16HBE)were cultured and divided into 4 groups:control group,IL-13 group,IL-13+SP group[IL-13+sensory neuropeptide substance P(SP)]and IL-13+WIN 62,577 group.16HBE cells were treated with IL-13(25 ng/ml)for 48 hours.After 48 hours,the IL-13+SP group was treated with sensory neuropeptide SP(10 nmol/L)for 1 hour,and the IL-13+WIN 62,577 group was treated with WIN 62,577(10 nmol/L)for 1 hour,respectively.The proliferation ability,reactive oxygen species(ROS)level,malondialdehyde(MDA)content and total superoxide dismutase(SOD)activity in each group were detected by corresponding kits.Results Compared with the control group,cell proliferation ability reduced(P<0.001),ROS level increased(P=0.001),MDA content increased(P<0.001),and SOD activity reduced(P<0.001)in the IL-13 group.Compared with the IL-13 group,the cell proliferation ability reduced(P=0.016),the ROS level increased(P=0.031),the MDA content increased(P<0.001),and the SOD activity decreased(P=0.011)after sensory neuropeptide SP stimulation.However,the intervention of WIN 62,577 could inhibit the decrease of cell proliferation(P=0.018),the increase of ROS level(P=0.018),the increase of MDA content(P<0.001),and the decrease of SOD activity(P=0.001)in human bronchial epithelial cells induced by IL-13.Conclusion Sensory neuropeptide SP could aggravate oxidative stress induced by IL-13 in human bronchial epithelial cells,while the sensory neuropeptide SP receptor NK-1R antagonist WIN 62,577 could alleviate oxidative stress induced by IL-13 in human bronchial epithelial cells.
作者
王植嘉
尚云晓
Wang Zhijia;Shang Yunxiao(Department of Pediatrics,Shengjing Hospital of China Medical University,Shenyang 110004,China)
出处
《中国小儿急救医学》
CAS
2020年第7期516-520,共5页
Chinese Pediatric Emergency Medicine
基金
辽宁省高等学校创新团队支持计划(2018479)。
作者简介
通信作者:尚云晓,Email:shangyunx@sina.com。
