摘要
为研究影响糜子幼胚愈伤组织诱导分化的因素,优化培养条件,提高糜子幼胚培养效率。以‘陕糜1号’和‘陕糜2号’为材料,研究低温预处理时间、激素种类及质量浓度对糜子幼胚愈伤组织诱导、分化和再生的影响。结果表明,4℃低温预处理3 d能显著提高糜子的幼胚愈伤组织诱导率;2种糜子最适宜的诱导培养基均为MS+3.0 mg/L 2,4-D。添加一定质量浓度的KT,可以改善愈伤组织质量,但是2种糜子的愈伤组织诱导率有显著的基因型差异。可见,2种糜子最适宜的分化培养基均为MS+1.0 mg/L 6-BA+0.1 mg/L NAA,且绿点率均在80%以上。
This study is to optimize the culture conditions of callus induction and differentiation for improving the culture efficiency of proso millet immature embryos.With Shaanmi No.1(SM1)and Shaanmi No.2(SM2)as experimental materials,the effects of low temperature,type and concentrations of hormones on induction,differentiation and regeneration of calli from immature embryos of proso millet were studied.The results showed that pretreatment of 4℃low temperature for 3 days significantly increased frequency of callus induction from proso millet immature embryos.The suitable callus induction medium for two proso millet varieties was MS medium with 2,4-D(3.0 mg/L).Addition of a certain concentration of kinetin(KT)could improve callus quality,and callus induction frequency showed significantly genotype differences between the two varieties.The most suitable differentiation medium for both proso millets was MS+1.0 mg/L 6-BA+0.1 mg/L NAA(0.1 mg/L),and the green spot rate was above 80%.
作者
吴会琴
王娜
黄梦迪
刘蓓蓓
杨璞
高小丽
WU Huiqin;WANG Na;HUANG Mengdi;LIU Beibei;YANG Pu;GAO Xiaoli(State Key Laboratory of Crop Stress Biology for Arid Areas,College of Agronomy,Northwest A&F University,Yangling Shaanxi 712100,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2020年第8期1177-1185,共9页
Acta Agriculturae Boreali-occidentalis Sinica
基金
陕西省重点研发计划(2018TSCXL-NY-03-01)
国家谷子高粱产业技术体系(CARS-06-13.5-A26)
陕西省农业科技创新转化项目(NYKJ-2018-YL19)。
关键词
糜子
幼胚
愈伤组织诱导
再生体系
Proso millet(Panicum miliaceum L.)
Immature embryos
Callus induction
Regeneration system
作者简介
第一作者:吴会琴,女,硕士研究生,从事旱区高效农作制度与作物栽培研究,E-mail:chenhun1993@163.com;通讯作者:高小丽,女,博士,硕士生导师,主要从事小杂粮栽培、育种及产业化开发研究,E-mail:gao2123@nwsuaf.edu.cn。
