摘要
[目的]为了了解山东省滨州地区及周边规模化牛场支原体流行的基本情况,[方法]对该区域14个规模化奶牛场和肉牛场进行了血清采集,采用牛支原体IgG抗体ELISA检测方法进行牛支原体血清抗体检测,并使用SPSS 20进行卡方统计学检验。[结果]山东省规模化牛场的牛支原体IgG抗体总阳性率为77.86%,奶牛场的阳性率为74.29%,肉牛场的阳性率为81.43%,奶牛场牛支原体IgG阳性率与肉牛场的统计学差异不显著(P>0.05);奶牛场不同场区,肉牛场不同场区之间,牛支原体的IgG阳性率差异显著(P<0.05)。[结论]山东省滨州地区及周边规模化牛场存在不同程度的牛支原体感染,牛支原体感染可能成为危害国内规模化奶牛场奶牛健康的主要疫病之一。
[Objective]The authors want to understand the prevalence of Mycoplasma bovis in large-scale cattle farms in Shandong Binzhou area.[Methods]Some serum samples were collected from 14 large-scale dairy farms and beef farms in this area.The serum antibody of Mycoplasma bovis was detected by indirect ELISA Kit with IgG antibody of Mycoplasma bovis,and chi square statistical test was performed by SPSS 20.[Results]The results showed that the total positive rate of Mycoplasma bovis IgG antibody was 77.86%,and there was no significant difference between the positive rate of Mycoplasma in large-scale dairy farms(74.29%)and beef farms(81.43%)(P>0.05).There was a significant difference in the IgG positive rate of Mycoplasma bovis between different dairy farm areas and beef farm areas(P<0.05).[Conclusion]There are different degrees of Mycoplasma bovis infection in different farms in Binzhou Shandong province.Mycoplasma bovis infection may become one of the main diseases that harm the health of domestic large-scale cattle farms.
作者
李书光
肖跃强
程立坤
赵家磊
李来永
刘吉山
LI Shu-guang;XIAO Yue-qiang;CHENG Li-kun;ZHAO Jia-lei;LI Lai-yong;LIU Ji-shan(Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, Shandong 256600;Qingdao Agricultural University Qingdao, Shandong 266109;Shandong Binzhou Research, Development and Promotion Center for Livestock and Poultry Propolis Vaccine, Binzhou, Shandong 256600;Shandong Lvdu Biotechnology Co., Ltd., Binzhou, Shandong 256600;Grassland Supervision Station, Hekou District, Dongying City, Dongying, Shandong 257200)
出处
《中国牛业科学》
2020年第4期30-32,59,共4页
China Cattle Science
基金
山东省农业产业技术体系牛创新团队项目(SDAIT-09-12)。
作者简介
李书光(1982—),男,副研究员,博士,主要从事分子细菌学研究和生物制品学研究,E-mail:lshug@163.com;通讯作者:刘吉山(1975—),男,研究员,博士,主要从事兽医临床技术诊疗与研究。
