摘要
目的:采用整合药理学方法预测清肺排毒汤治疗新型冠状病毒肺炎(COVID-19)的现代功效及作用机制,为其进一步开发研究及临床应用提供参考。方法:依托中医药整合药理学研究平台V2. 0,预测清肺排毒汤治疗新型冠状病毒肺炎的的关键靶标和作用通路,绘制"方剂-中药-疾病-关键靶标-作用通路"多维网络图,中西医结合探讨其临床作用及作用机制。结果:预测得到的清肺排毒汤排名前20的核心靶标如细胞肿瘤抗原p53(tp53),蛋白激酶B1(Akt1)以及系列核因子核转录因子-κB(NK-κB)p105亚基(NFKB1),核因子p65亚基(RELA),人NK-κB抑制蛋白α(NFKBIA)等大多与肺部损伤相关。推测清肺排毒汤可能通过调控白细胞介素(IL)信号通路、肾上腺素受体,C型凝集素域家族7成员A(CLEC7A)/炎症途径,磷脂酰肌醇-3激酶(PI3K)/蛋白激酶B(Akt)炎症信号传导通路,tp53调节的DNA修复基因的转录等相关信号通路,针对肺炎、发烧咳嗽等症状起治疗作用。研究结果表明,清肺排毒汤具有抗炎、抗病毒、增强免疫力、抑制肺纤维化发展、保护心肺、治疗哮喘、调理胃肠道等临床疗效多方面功效,且合方之原方间呈现出较好的协同作用又各有侧重。结论:预测清肺排毒汤可通过多途径、多靶点、多环节防治COVID-19,且合方功效优于原方,适用于COVID-19各个病程的治疗。本研究结果为预测清肺排毒汤的临床应用提供理论依据,同时为中医药防治现代传染性疾病提供研究思路。
Objective: Potential targets and pathways of Qingfei Paidu decoction(QFPD)for treating coronavirus disease-2019(COVID-19)were analyzed based on the integrative pharmacology,the efficacy and material basis was predicted. This study provide a reference for the development and clinical application of QFPD. Method: Based on the integrative pharmacology of traditional Chinese medicine(TCMIP V2. 0),the key targets and pathways of the intervention of QFPD on COVID-19 were enriched,the interaction network of "formula-herb-disease-targets-pathways" was constructed to explored the molecular mechanism of QFPD for the treatment of COVID-19. Result: The research results show that key-targets such as cell tumor antigen p53(tp53),protein kinase B1(Akt1),Nuclear factor nuclear transcription factor-κB(NF-κB)p105 subunit(NFKB1),nuclear factor p65 subunit(RELA),human NF-κB inhibited protein α(NFKBIA),ect. Closely associated with lung damage. The pathways such as interleukin signaling,adrenoceptors,7 members of the family of c-type lectin domains A(CLEC7 A)/inflammasome pathway,phosphoinositide-3-kinase(PI3 K)/protein kinase B(Akt)inflammatory signaling pathway,tp53 regulates transcription of DNA repair ect. may be the key pathways related with QFPD’s effect on the treatment of COVID-19 accompany with lung injury,fever,cough and other symptoms. The results show that QFPD has many clinical effects,such as anti-inflammatory,anti-virus,strengthening immunity,inhibit the development of pulmonary fibrosis,protecting heart and lungs,treating asthma,regulating gastrointestinal tract,etc. In addition,there is a good synergism between the original prescription and the combined prescription,and each original prescription has its own emphasiscan prevention and treatment of COVID-19. Conclusion: QFPD plays a role in balancing immunity and eliminating inflammation,and it can treat COVID-19 by multi-pathway,multi-channel,multi-target and multi-link. This study also provides a new idea for the research of prevention and treatment of modern infectious diseases by use the traditional Chinese medicine.
作者
彭修娟
杨新杰
许刚
陈衍斌
杨长花
龚伟玲
韩冬
刘峰
PENG Xiu-juan;YANG Xin-jie;XU Gang;CHEN Yan-bin;YANG Chang-hua;GONG Wei-ling;HAN Dong;LIU Feng(Shaanxi Institute of International Trade&Commerce,Xi'an 712046,China;Shaanxi University of Chinese Medicine,Xi'an 712046,China;Shaanxi Buchang Pharmaceutical Co.Ltd.,Xi'an 710075,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2020年第16期6-13,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
陕西省中药绿色制造技术协同创新中心项目(陕教[2018]12号)
陕西高校青年科技创新团队项目(陕教[2019]90号)
陕西省中药产业技术创新服务共享平台项目(2018PF-07)。
关键词
新型冠状病毒肺炎
清肺排毒汤
临床功效
作用机制
整合药理学
coronavirus disease-2019(COVID-19)
Qingfei Paidu decoction
clinical efficacy
mechanism
integrative pharmacology
作者简介
第一作者:彭修娟,硕士,副教授,从事中药药效物质基础研究,Tel:029-33694603,E-mail:610348434@qq.com;通信作者:刘峰,博士,主任药师,从事经典名方开发研究,Tel:029-33694603,E-mail:liufeng1720@163.com。
