摘要
【目的】腐皮病是绿海龟(Chelonia mydas)龟苗培育中的常见病,传染性极强,发病率和死亡率高。为确定病因及治疗方法,从病龟鳍状肢的腐皮组织中分离病原菌。【方法】从症状明显的稚龟四肢病灶中分离纯化得到疑似病原菌,编号CMRT91026,开展形态特征、生理生化特性、16SrRNA鉴定和系统发育树构建及相关药物敏感试验。【结果】该菌能在TCBS培养基上生长,菌落呈黄色,为革兰氏阴性短杆菌;在1%NaCl葡萄糖磷酸盐胨水、蔗糖、甘露糖和赖氨酸脱羧酶上的生化特性均显示阳性,并且在3%~10%NaCl胨水均能生长;与NCBI上的溶藻弧菌(Vibrio alginolyticus)同源性达100%。药敏结果显示,该菌对恩诺沙星、萘啶酸、左氧氟沙星、米诺环素、头孢吡肟和头孢曲松敏感;对多西环素、氟苯尼考、妥布霉素、大观霉素、诺氟沙星和氨曲南呈中介;对新霉素、复方新诺明、阿莫西林、庆大霉素、丁胺卡那霉素等15种药物有耐药性。【结论】该菌株鉴定为溶藻弧菌,对喹诺酮类药物(如恩诺沙星)、四环素类药物(如米诺环素)及头孢类(如头孢曲松)等药物较为敏感。试验结果为绿海龟稚龟溶藻弧菌病的诊断及病害防控提供了一定参考。
【Objective】Rotten skin is a common disease in hatchling breeding of green sea turtles(Chelonia mydas),with high infectivity,morbidity and mortality.In order to determine the etiology and treatment of the disease,a pathogenic bacterial was isolated from the rotten tissue of flipper of diseased C.mydas.【Method】Suspected pathogens were isolated and obtained from limb lesions of diseased larval turtle and marked as CMRT91026.The morphological,physiological and biochemical characteristics as well as drug susceptibility were analysed.The phylogenetic tree was established on the basis of the identified 16S rRNA.【Result】The results showed that the isolated strain could grow on TCBS medium and the colony was yellow.The physiological and biochemical analysis indicated that the strain CMRT91026 was Gram-negative Brevi bacterium.The biochemical characteristics of the strain CMRT91026 on 1%NaCl phosphate glucose peptone water,sucrose,mannose and lysine decarboxylase media presented positive and it could grow in 3%-10%peptone.The homology of 16S rRNA among CMRT91026 and other typical Vibrio alginolyticus on NCBI was 100%.Thus,strain CMRT91026 was identified as V.alginolyticus.The antibiotic susceptibility test showed that strain CMRT91026 was sensitive to enrofloxacin,nalidixic acid,levofloxacin,minocycline,cefepime and ceftriaxone.It exhibited intermediate sensitivity to doxycycline,flurfenicol,tobramycin,spectinomycin,norfloxacin,aztreonam and resistance to 15 drugs such as neomycin,sulfamethoxazole compound,amoxicillin,gentamicin,amikacin and etc.【Conclusion】The strain is identified as V.alginolyticus and it is sensitive to quinolones(enrofloxacin),tetracyclines(minocycline)and cephalosporins(ceftriaxone).The test results contribute to the diagnosis and prevention of V.alginolyticus disease in larval green sea turtles.
作者
叶明彬
陈华灵
冯国清
梁志凌
端金霞
刘振兴
观玉安
YE Mingbin;CHEN Hualing;FENG Guoqing;LIANG Zhiling;DUAN Jinxia;LIU Zhenxing;GUAN Yu'an(National Huidong Sea Turtle Reserve Management Bureau,Huidong 516359,China;Institute of Animal Health,Guangdong Academy of Agrioultural Sciences,Guangzhou 510640,China)
出处
《广东农业科学》
CAS
2020年第6期97-105,共9页
Guangdong Agricultural Sciences
基金
广东省海洋经济发展专项(GDNRC2020040)。
关键词
绿海龟
种苗培育
溶藻弧菌
16SrRNA分析
药敏试验
Chelonia mydas
hatchling breeding
Vibrio alginolyticus
16SrRNA analysis
drug susceptibility
作者简介
叶明彬(1976—),男,高级工程师,研究方向为绿海龟人工繁育及保护,E-mail:454134217@qq.com。
