摘要
根系建成(RSA,Root system architecture)决定根系系统的构成,在作物生长发育过程中起着不可替代的作用。解析小麦根系建成遗传机制、选育具有较好根系建成的品种对于小麦高产、抗逆育种具有十分重要的意义。全基因组关联分析(GWAS)是解析小麦复杂数量遗传性状遗传机制的有效方法。本研究基于全基因组关联分析方法,发掘根系建成相关性状关联位点,以期为小麦根系建成分子育种提供参考。对160份来自于河南和山东等地的小麦品种根系建成相关性状(总根长、总根表面积、总根体积、平均根直径和根尖数)进行统计评价,并结合660K SNP芯片数据进行全基因组关联分析。检测到23个关联位点,分布于1A、2A、2B、3B、4A、5A、5B、5D、6A、6B和7B染色体上,解释7.2%~12.8%的表型变异。其中,11个位点与已报道的位点一致,其他12个位点为新的位点。本研究对于解析根系建成遗传机制,选育高产、抗逆小麦品种具有重要意义。
The root system architecture(RSA) determines the composition of the root system and plays an irreplaceable role in crop growth and development. It is of great significance to analyze the genetic mechanism of wheat root architecture and the selection of varieties with better RSA traits in breeding for elite wheat varieties. Genome-wide association study(GWAS) is an effective method to analyze the genetic mechanism of complicated quantitative traits(i.e. RSA) in wheat. In the study,GWAS for RSA-related traits(total root length,total root surface area,total root volume,average root diameter and number of root tips) were conducted in 160 wheat accessions from Henan and Shandong,which were previously genotyped by wheat 660 K SNP chip. Twenty-three loci associated with RSA related traits were detected from chromosomes 1 A,2 A,2 B,3 B,4 A,5 A,5 B,5 D,6 A,6 B and 7 B,and each explained the phenotypic variation variable from 7.2% to 12.8%. Among them,eleven loci are found to coincide with the formerly-reported loci,and the remaining 12 loci are newly identified. Taken together,this study is of significance to uncover the genetic structure of the root architecture with a potential in future breeding of high-yield and stress-resistant wheat cultivars.
作者
陈贵菊
靳义荣
刘彩云
贾德新
樊庆琦
刘金栋
刘鹏
CHEN Gui-ju;JIN Yi-rong;LIU Cai-yun;JIA De-xin;FAN Qing-qi;LIU Jin-dong;LIU Peng(Jining Academy of Agricultural Sciences,Jining 272031;Dezhou Academy of Agricultural Sciences,Dezhou 253015;Crop Research Instituter,Shandong Academy of Agricultural Sciences,Jinan 250100;Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences(CAAS),Shenzhen 518124)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2020年第4期975-983,共9页
Journal of Plant Genetic Resources
基金
山东省重点研发计划项目(2017GNC10107)
山东省西部经济隆起带基层科技人才支持计划项目(XB2018FW029)
国家重点研发计划项目(2017YFD0100600)
山东省现代农业产业技术体系小麦创新团队建设项目(SDAIT-01-17)
山东省农业科学院农业科技创新工程项目(CXGC2016B01)
山东省自然科学基金项目(ZR2017BC015)
德州市农业科学院资助项目(2019-01)。
作者简介
第一作者:陈贵菊,研究方向为小麦遗传育种,E-mail:cgj1020@163.com;第一作者:靳义荣;通信作者:刘金栋,研究方向为小麦遗传育种,E-mail:liujindong_1990@163.com;通信作者:刘鹏,研究方向为小麦遗传育种,E-mail:liup9@163.com。
