摘要
本文建立了一种利用大孔树脂分离富集缺陷假单胞菌HD13发酵液中抑制水稻纹枯病菌活性物质的方法。以水稻纹枯病菌为测试病原菌,采用缺陷假单胞菌HD13发酵液考察D101、LX-60、XDA-6、LSA-12、XDA-8G、LX-17共6种大孔树脂对HD13菌株发酵液中抑制水稻纹枯病菌活性物质的分离效果。结果表明:XDA-8G树脂对其有很好的分离和富集作用,主要集中在40%乙醇洗脱液中,该方法成本低、操作简单、快速,可为后续大量发酵提供实践依据。
In this paper,a method for separating and enriching inhibiting active substances of Thanatephorus cucumeris from the fermentation broth of Pseudomonasdiminita HD13 by using macroporous resin was established.Using Thanatephorus cucumeris as the test pathogen,six macroporous resins(D101,LX-60,XDA-6,LSA-12,XDA-8 G and LX-17)were used to separate the inhibiting active substances of Thanatephorus cucumeris from the fermentation broth of Pseudomonasdiminita HD13.The results showed that XDA-8 G resin had a good separation and enrichment effect.The inhibiting active substances were concentrated in the 40%alcohol eluent.The method has low cost,simple and fast operation,and can provide practical basis for subsequent large-scale fermentation.
作者
杨兴变
郑雯
何珺
康冀川
李洪庆
YANG Xingbian;ZHEN Wen;HE Jun;KANG Jichuan;LI Hongqing(Engineering and Research Center of Guizhou Province of China,Guizhou University,Guiyang,Guizhou 550025,China;College of Pharmacy,Guizhou University,Guiyang,Guizhou 550025,China;College of Chemistry and Environmental Science,Guizhou Minzu University,Guiyang,Guizhou 550025,China)
出处
《山地农业生物学报》
2020年第3期85-88,共4页
Journal of Mountain Agriculture and Biology
基金
贵州大学实验室开放项目(SYSKF2019-096)
贵州大学实验室开放项目(SYSKF2017-2-071)
贵州大学教育部重点实验室(工程中心)开放课题基金(GZUKEY20160703)。
关键词
缺陷假单胞菌发酵液
活性物质
大孔树脂
水稻纹枯病菌
the fermentation broth of Pseudomonasdiminita
active substances
macroporous resin
Thanatephorus cucumeris
作者简介
通讯作者:康冀川(1962-),男,博士,教授,主要从事分子生物学和真菌学研究,E-mail:jckang@/gzu.edu.cn。
