摘要
为揭示河口湿地微生态环境因子对土壤微生物群落的影响,在辽东湾小凌河口设置5条监测样带,每条监测样带设置4个监测样点,其中1条(样带G)位于围海养殖圩堤附近,另外4条(样带A、B、C、D)依次从圩堤向河口水域靠近,采集了不同样带的土壤样品,利用磷脂脂肪酸法分析其土壤微生物群落结构分异特征。结果表明:养殖塘圩堤显著改变了湿地土壤微生态环境特征和微生物群落结构,圩堤以外湿地土壤微生物丰度沿样带环境梯度先升高后降低,最高值(样带C)比近河口水域区域(样带D)细菌、革兰氏阳性细菌、革兰氏阴性细菌和真菌丰度分别增加了33.33%、22.00%、50.07%和35.65%;土壤微生物群落结构与钾离子(K+)、钙离子(Ca2+)、总氮、镁离子(Mg2+)及硝态氮呈显著相关关系;方差分解分析显示,盐基离子累积可以解释微生物群落结构变异的72.9%,随着土壤中镁钙离子比(Mg2+/Ca2+)的升高,真菌、放线菌和革兰氏阳性细菌丰度呈幂函数显著降低。因此,河口湿地围海养殖和湿地盐基离子的变化对土壤微生物群落结构产生了深刻影响。
To reveal the response of soil microbial community to changes of micro-ecological environment,five monitoring transects were set up in Xiaolinghe estuarine wetland of Liaodong Bay,with four monitoring plots in each transect.The transect G was close to the polder embankment of the culture pond,while the other four transects(Transects A,B,C,D)were situated in proper sequence towards the water area with an interval of 100 m.Phospholipid fatty acids(PLFA)analysis was applied to determine soil microbial community structure.Results showed that the polder embankment significantly affected soil microbial community structure and micro-ecological environmental factors.Abundance of soil microbes of the tidal flat outside the polder embankment increased first and then decreased along the direction from the shore towards the water area.Total bacteria,Gram-positive bacteria,Gram-negative bacteria and fungi of transect C were significantly 33.33%,22.00%,50.07%and 35.65%higher than that in transect D(close to water area).PLFA signatures were significantly correlated with soil K+,Ca2+,total N,Mg2+and NO3--N concentrations.Results of variation partitioning analysis showed that soil base ion contents together explained 72.9%of the variation of microbial community structure.Abundance of fungi,actino-myces and Gram-positive bacteria significantly decreased by means of a negative power function with increasing Mg2+/Ca2+.In summary,polder embankment and base ion content had significant effects on soil microbial community structure.
作者
张广帅
闫吉顺
赵全民
于永海
于秀波
张全军
蔡悦荫
宫玮
ZHANG Guang-shuai;YAN Ji-shun;ZHAO Quan-min;YU Yong-hai;YU Xiu-bo;ZHANG Quan-jun;CAI Yue-yin;GONG Wei(National Marine Environmental Monitoring Center,Dalian 116023,Liaoning,China;Key Laboratory of Ecosystem Network Observation and Modeling,Institute of Geographic Sciences and Natural Resources Research,Chinese Academy of Sciences,Beijing 100101,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2020年第7期2283-2291,共9页
Chinese Journal of Ecology
基金
国家海洋环境监测中心博士启动基金(2019-A-03)
阿拉善SEE青年学者项目(ST202002015)
辽宁省博士科研启动基金(2019-BS-091)资助。
关键词
河口湿地
土壤微生物群落结构
盐基离子
方差分解分析
estuarine wetland
soil microbial community structure
base ion
variation partitioning analysis
作者简介
张广帅,男,1989年生,博士,助理研究员,主要研究方向为滨海湿地生态演变与修复,海洋生态评估与补偿技术。E-mail:zhanggs.nmemc@foxmail.com;通讯作者:闫吉顺,E-mail:jsyan@nmemc.org.cn。
