摘要
目的:观察C57小鼠急性肝损伤(Acute liver injury,ALI)中线粒体自噬相关蛋白Pink1/Parkin表达的变化及意义。方法:领取28只小鼠,随机分为对照组、ALI 1 d组、ALI 4 d组、ALI 7 d组。分别检测血清谷氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)的变化;肝组织病理变化用H&E染色观察;Real Time quality PCR检测Pink1/Parkin在m RNA水平的表达变化;进一步利用Western Blot方法分别检测肝组织中Pink1/Parkin蛋白水平的表达变化。结果:ALI组1 d、4 d、7 d组与对照组相比,ALT、AST表达显著升高,具有统计学意义(P<0.05);肝组织病理学检查ALI组1 d和4 d组中,释放出大量的炎性细胞,肝细胞出现大面积坏死,7 d组肝细胞坏死与1 d和4 d比较明显缓解;m RNA水平检测Pink1/Parkin在ALI 1 d(4.79/1.82倍)、4 d(1.74/4.17倍)和7 d(2.91/1.25倍)与对照组相比表达水平明显升高(P<0.05);Pink1/Parkin在蛋白水平检测时ALI 1 d(2.03/1.82倍)、4 d(1.78/4.17倍)和7 d(1.25/1.25倍)时肝组织内表达也显著增加,差异具有统计学意义(P<0.05)。结论:急性肝损伤状态下Pink1/Parkin在m RNA和蛋白水平表达升高,且其表达量随着肝脏的损伤缓解而表达水平降低,提示Pink1/Parkin可能对于治疗急性肝损伤具有重要意义。
Objective:To observe the change of mitochondrial autophagy-associated protein Pink1/Parkin expression in acute liver injury(ALI)of C57 mice and its significance.Methods:Twenty-eight mice were randomly divided into control group,ALI 1 d group,ALI 4 d group,and ALI 7 d group.The change of serum glutamate aminotransferase(ALT)and aspartate aminotransferase(AST)were detected respectively.The pathological changes of liver tissue were observed by H&E staining.The expression of Pink1/Parkin in mRNA level were detected by Real Time quality PCR.Next step,the expression of Pink1/Parkin in protein level of liver tissue were detected by the Western blot methods.Results:The level of ALT and AST in ALI groups at 1 d,4 d and 7 d were significantly higher than in the sham group(P<0.05).H&E staining showed that a mass of inflammatory cells infiltration and cells degeneration were observed in ALI groups,and hepatocyte necrosis in 7 d group were significantly relieved than 1 d and 4 d.The result of real time quality-PCR indicated that the level of Pink1/Parkin enhanced significantly at 1 d(4.79/1.82 fold),4 d(1.74/4.17 fold)and 7 d(2.91/1.25 fold)in ALI groups compared with sham group(P<0.05).Western blot results showed that the expression of Pink1/Parkin were detected in the extracted protein of liver tissue which were significantly increased in the group of ALI 1 d(2.03/1.82 fold),4 d(1.78/4.17 fold)and 7 d(1.25/1.25 fold)compared with sham group(P<0.05).Conclusion:Pink1 and Parkin were highly expression in protein and mRNA levels of acute liver injury,and its expression level were decreased with the relief of liver tissue damage.In addition,Pink1/Parkin could be have a potential significance for the treatment of acute liver injury.
作者
拜云虎
吴菲菲
孔二亮
王亚云
王全晖
杨雁灵
BAI Yun-hu;WU Fei-fei;KONG Er-liang;WANG Ya-yun;WANG Quan-hui;YANG Yan-ling(Department of General Surgery,Zhengzhou Hospital of the 988 Hospital of the People's Liberation Army,Zhengzhou,Henan,450000,China;The Air Force Military Medical University Department of Anatomy,Xi'an,Shaanxi,710032,China;Department of Anesthesiology,Zhengzhou Hospital of the 988 Hospital of PLA,Zhengzhou,Henan,450000,China;Air Force Department of Hepatobiliary and Pancreatic Surgery,the First Affiliated Hospital of Military Medical University,Xi'an,Shaanxi,710032,China)
出处
《现代生物医学进展》
CAS
2020年第9期1648-1651,1630,共5页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81470843)。
作者简介
拜云虎,硕士、住院医师,研究方向:急性肝损伤和肝癌的机制研究,E-mail:byunhu@163.com;通讯作者:杨雁灵,副教授、博士、硕士生导师,研究方向:代谢性脑病机制研究,E-mail:yangylfmmu@163.com。
