摘要
目的探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)患者鼠类肉瘤滤过性毒菌致癌同源体B(v-Raf murine sarcoma viral oncogene homolog B,BRAF)V600E基因突变及超声特征与颈部淋巴结转移的关系。方法PTC患者34例,其中有颈部淋巴结转移11例,无颈部淋巴结转移23例;患者术前均行超声检查,记录肿瘤直径、边缘、纵横比、有无钙化等特征;取手术切除的PTC组织和癌旁组织各34份,采用DNA测序法检测BRAF V600E基因突变情况。比较有、无颈部淋巴结患者BRAF V600E基因突变率及超声声像特征。结果PTC组织BRAF V600E基因突变率(52.94%)高于癌旁组织(0)(P<0.05);有颈部淋巴结转移者超声声像示肿瘤钙化比率(81.82%)高于无颈部淋巴结转移者(43.48%)(P<0.05),肿瘤直径[(21.36±7.54)mm]、BRAF V600E基因突变率(45.45%)、纵横比>1比率(63.64%)和肿瘤边缘不光整比率(72.73%)与无颈部淋巴结转移者[(18.29±6.93)mm、56.52%、78.26%、69.57%]比较差异无统计学意义(P>0.05)。结论PTC组织BRAF V600E基因突变率较高,其与颈部淋巴结转移无明显相关性,超声声像示肿瘤钙化者可能更易出现颈部淋巴结转移。
Objective To investigate the correlations of v-Raf murine sarcoma viral oncogene homolog B(BRAF)V600 E gene mutation and the ultrasonographic features with cervical lymphatic metastasis in patients with papillary thyroid carcinoma(PTC).Methods Thirty-four PTC patients including 11 patients with cervical lymphatic metastasis and 23 patients with no cervical lymphatic metastasis received ultrasound examination before operation to record the tumor diameter,margin,aspect ratio and calcification.The fresh surgical specimens of PTC tissues and adjacent tissues were collected,and BRAF V600 E mutations were analyzed by DNA sequencing.The mutation rates and ultrasonographic features were compared between the patients with and without cervical lymphatic metastasis.Results The mutation rate of BRAF V 600 E gene was higher in PTC tissues(52.94%)than that in adjcent tissues(0)(P<0.05).Calcification was more frequently detected in PTC patients with cervical lymphatic metastasis(81.82%)than in those without cervical lymphatic metastasis(43.48%)(P<0.05),while the tumor diameter((21.36±7.54)mm vs.(18.29±6.93)mm),BRAF V600 E mutation rate(45.45%vs.56.52%),percentage of aspect ratio>1(63.64%vs.78.26%),and percentage of ill-defined margin(72.73%vs.69.57%)showed no significant differences between patients with and without cervical lymphatic metastasis(P>0.05).Conclusion BRAF V600 E mutation is high in PTC tissues but it is independent of cervical lymphatic metastasis.Calcification on ultrasound is correlated with cervical lymphatic metastasis in PTC patients.
作者
张鹏
翟渊鹏
李潜
ZHANG Peng;ZHAI Yuanpeng;LI Qian(Department of Strategy and Development,Affiliated Cancer Hospital of Zhengzhou University,Zhengzhou 450008,China;Department of Ultrasound,Affiliated Cancer Hospital of Zhengzhou University,Zhengzhou 450008,China)
出处
《中华实用诊断与治疗杂志》
2020年第6期611-614,共4页
Journal of Chinese Practical Diagnosis and Therapy
基金
河南省科技攻关项目(172102410011)。
作者简介
通信作者:李潜,E-mail:754427296@qq.com。
