摘要
建立了离子色谱法测定磺胺氯吡嗪钠中钠离子含量的方法。试验采用Dionex IonPac CS12A(4 mm×250 mm)阳离子交换色谱柱,以甲烷磺酸溶液为淋洗液,流速为1.0 mL/min,柱温为30℃,抑制型电导检测器。试验结果表明钠离子与常见成盐阳离子分离良好,在0.2~15μg/mL浓度范围内呈良好的线性关系(r2=1.0000),加样回收率为100.7%(n=9,RSD=3.0%)。建立的方法准确、灵敏、专属性好,可用于测定磺胺氯吡嗪钠中钠离子的含量。
A method for content determination of sodium ion in sulfachloropyrazine sodium was developed by ion chromatography with inhibitory electrical conductivity detector.Analysis was performed on a Dionex IonPac CS12A column(4 mm×250 mm),using methane sulfonic acid solution as the mobile phase.The flow rate was 1.0 mL/min and the column temperature was set at 30℃.Results showed that a good separation was accepted between sodium ion and other conventional cations.The standard curve was linear in the range of 0.2~15μg/mL(r2=1.0000).The average recovery was 100.7%(n=9,RSD=3.0%).In conclusion,this method is accurate,sensitive and specific for the determination of sodium ion in sulfachloropyrazine sodium.
作者
杨星
高立红
龚旭昊
马秋冉
董玲玲
赵富华
于晓辉
YANG Xing;GAO Li-hong;GONG Xu-hao;MA Qiu-ran;DONG Ling-ling;ZHAO Fu-hua;YU Xiao-hui(China Institute of Veterinary Drug Control,Beijing,100081,China;Thermo Fisher Scientific,Beijing,100102 China)
出处
《中国兽药杂志》
2020年第6期42-48,共7页
Chinese Journal of Veterinary Drug
关键词
离子色谱
磺胺氯吡嗪钠
钠离子
ion chromatography
sulfachloropyrazine sodium
sodium ion
作者简介
杨星,助理研究员,从事兽用化学药品检验工作;通讯作者:于晓辉,E-mail:Shuilon0220@sina.com。
