摘要
通过响应面法对乙醇水溶液浸提芦苇叶黄酮的工艺条件进行优化,以提高黄酮的产率;通过扫描电镜探究浸提法对芦苇叶结构的影响,并从植物结构的角度对提取过程机理进行探究。以料液比、加热温度、加热时间、乙醇体积分数作为影响黄酮提取的单因素进行试验,建立四因素三水平的响应面数据模型,获得最佳工艺条件为:加热温度为59.9℃,加热时间为1.995 h,料液比为1:25.13(g/mL),乙醇体积分数为59.7%,此时,黄酮的收率为24.310 mg/g;由扫描电镜图分析得到浸提法对芦苇的结构具有很强的影响作用,通过改变芦苇叶片的结构将其中的物质提取出来。
The optimum technological conditions of flavonoids extracted from ethanol aqueous solution were optimized by using the response surface method in order to achieve the maximum yield of flavonoids.The effect of leaching formulation on the structure of Reed leaves was explored by using scanning electron microscope,and the mechanism of extraction process was investigated from the point of view of plant structure.Based on the single factor test of the material liquid ratio,heating temperature,heating time and ethanol concentration,the four-factor three-level response surface data model was established.The optimum technological conditions were heating temperature 59.9℃,heating time 1.995 h,material liquid ratio 1∶25.13 g/mL,and ethanol concentration 59.7 per cent,with thhe yield of flavonoids being 24.310 mg/g.By scanning electron microscopy,the leaching formula had a strong effect on the structure of reed,and the material was extracted by changing the structure of reed leaves.
作者
师晓强
彭效明
周润
唐银珊
陈虹杏
陈新宇
SHI Xiaoqiang;PENG Xiaoming;ZHOU Run;TANG Yinshan;CHEN Hongxing;CHEN Xinyu(School of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102600, China)
出处
《北京石油化工学院学报》
2020年第2期1-8,共8页
Journal of Beijing Institute of Petrochemical Technology
基金
国家青年科学基金项目(21406015,81703453)
北京石油化工学院校内学科平台建设项目(2018XK002,2019XK006)
北京市大兴区科技发展计划项目(KT201806402)
北京市教委科技计划一般项目(KM201810017002)
北京市优秀人才培养自主项目(201600002012G058)
北京石油化工学院大学生研究训练计划URT项目(2018J00041,2014J00141)。
关键词
浸提法
响应面设计
黄酮
芦苇叶
扫描电镜
extraction method
response surface design
flavonoids
reed leaves
scanning electron microscopy
作者简介
师晓强(1998—),男,本科在读,从事天然产物化学活性成分的分离纯化和制剂研究,E-mail:shi15011272946@gmail.com;通信联系人:彭效明(1983—),男,博士,硕士生导师,主要从事天然产物活性成分研究,E-mail:pengxiaoming@bipt.edu.cn。
